De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatumand grapefruit peel

BMC Genomics

Research Article

Maggie Levy¹  Noa Sela²  Radi Aly³  Clarita Kessler⁴  Samir Droby⁴  Vera Hershkovitz⁴  Ginat Rafael⁴  Leena Taha-Salaime⁵  Jia Liu⁶  Michael Wisniewski⁶ 

[1] Department of Plant Pathology and Microbiology, the Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, 76100, Revovot, Israel;Department of Plant Pathology and Weed Research, ARO, the Volcani Center, 50250, Bet Dagan, Israel;Department of Plant Pathology and Weed Research, the Volcani Center, Newe-Yaar Research Center, 30095, Ramat Yeshai, Israel;Department of Postharvest and Food Sciences, ARO, the Volcani Center, 50250, Bet Dagan, Israel;Department of Postharvest and Food Sciences, ARO, the Volcani Center, 50250, Bet Dagan, Israel;Department of Plant Pathology and Weed Research, the Volcani Center, Newe-Yaar Research Center, 30095, Ramat Yeshai, Israel;Department of Plant Pathology and Microbiology, the Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University of Jerusalem, 76100, Revovot, Israel;U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, 25430, Kearneysville, WV, USA;

关键词: Antagonist-fruit interaction;    Antagonist-pathogen interaction;    Biological agent;    RNA-seq;   

DOI  :  10.1186/1471-2164-14-168

 received in 2012-10-11, accepted in 2013-02-25,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe yeast Metschnikowia fructicola is an antagonist with biological control activity against postharvest diseases of several fruits. We performed a transcriptome analysis, using RNA-Seq technology, to examine the response of M. fructicola with citrus fruit and with the postharvest pathogen, Penicillium digitatum.ResultsMore than 26 million sequencing reads were assembled into 9,674 unigenes. Approximately 50% of the unigenes could be annotated based on homology matches in the NCBI database. Based on homology, sequences were annotated with a gene description, gene ontology (GO term), and clustered into functional groups. An analysis of differential expression when the yeast was interacting with the fruit vs. the pathogen revealed more than 250 genes with specific expression responses. In the antagonist-pathogen interaction, genes related to transmembrane, multidrug transport and to amino acid metabolism were induced. In the antagonist-fruit interaction, expression of genes involved in oxidative stress, iron homeostasis, zinc homeostasis, and lipid metabolism were induced. Patterns of gene expression in the two interactions were examined at the individual transcript level by quantitative real-time PCR analysis (RT-qPCR).ConclusionThis study provides new insight into the biology of the tritrophic interactions that occur in a biocontrol system such as the use of the yeast, M. fructicola for the control of green mold on citrus caused by P. digitatum.

【 授权许可】

Unknown   
© Hershkovitz et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.