Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1

BMC Complementary and Alternative Medicine

Research Article

Xin Kang¹  Yingli Wang¹  Zhiwei Fan²  Xiaoguang Lu²  Libin Zhan³  Jinhai Mu⁴  Chang Lv⁴  Siyao Li⁵  Chunyu Lv⁵  Robbie Ali⁶ 

[1] College (Institute) of Integrative Medicine, Dalian Medical University, 116044, Dalian, China;Department of Emergency Medicine, Zhongshan Hospital, Dalian University, 116001, Dalian, China;Department of Traditional Chinese Medicine, the Second Affiliated Hospital, Dalian Medical University, 116023, Dalian, China;Graduate School of Dalian University, 116044, Dalian, China;Graduate School of Zunyi Medical University, 563003, Zunyi, China;Windber Hospital Inc, Windber Medical Center, 15963, Windber, PA, USA;

关键词: Hemorrhagic shock;    Dai Huang Fu Zi Tang;    Endotoxin;    Small intestine;    Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP);    Zonula occludens (ZO)-1 protein;    Intestinal fatty acid binding protein (IFABP);   

DOI  :  10.1186/1472-6882-14-80

 received in 2013-11-25, accepted in 2014-02-24,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDai Huang Fu Zi Tang (DHFZT), an oriental herbal formula, has long been used clinically in treatment of intestinal obstruction, acute pancreatitis, cholecystalgia and chronic diarrhea for thousands of years. Recent studies have demonstrated that DHFZT can reduce intestinal pathological injury and the concentration of enterogenous endotoxin in an animal model. But the underlying mechanism has not been fully elucidated.MethodsSD male rats in adult were used to model HS and treated with DHFZT. The serum concentration of endotoxin were analyzed by dynamic turbidimetric method. In addition, the blood flow of small intestine were measured using laser speckle technique. Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP) and zonula occludens (ZO)-1 protein, intestinal fatty acid binding protein (IFABP) were measured by Western Blotting, RT-PCR, ELISA respectively.ResultsPresent study showed that DHFZT markedly elevated the blood flow of small intestine, protected the intestinal barrier function by up-regulating the expression of ZO-1 protein and down-regulating expression of p-VASP, and notely decreased serum concentration of IFABP and endotoxin in HS.ConclusionsThese results reveal that DHFZT improves intestinal blood flow, protects the intestinal barrier function, and ameliorates intestinal endotoxaemia in rats with HS.

【 授权许可】

CC BY   
© Lu et al.; licensee BioMed Central Ltd. 2014