Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1

BMC Complementary and Alternative Medicine

Jinhai Mu⁶  Siyao Li³  Chang Lv⁶  Robbie Ali⁵  Yingli Wang⁴  Zhiwei Fan²  Chunyu Lv³  Libin Zhan¹  Xin Kang⁴  Xiaoguang Lu² 

[1] Department of Traditional Chinese Medicine, the Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China;Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian 116001, China;Graduate School of Zunyi Medical University, Zunyi 563003, China;College (Institute) of Integrative Medicine, Dalian Medical University, Dalian 116044, China;Windber Hospital Inc, Windber Medical Center, Windber, PA 15963, USA;Graduate School of Dalian University, Dalian 116044, China

关键词: Intestinal fatty acid binding protein (IFABP);    Zonula occludens (ZO)-1 protein;    Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP);    Small intestine;    Endotoxin;    Dai Huang Fu Zi Tang;    Hemorrhagic shock;   

Others  :  1220243
DOI  :  10.1186/1472-6882-14-80

 received in 2013-11-25, accepted in 2014-02-24,  发布年份 2014

【 摘 要 】

Background

Dai Huang Fu Zi Tang (DHFZT), an oriental herbal formula, has long been used clinically in treatment of intestinal obstruction, acute pancreatitis, cholecystalgia and chronic diarrhea for thousands of years. Recent studies have demonstrated that DHFZT can reduce intestinal pathological injury and the concentration of enterogenous endotoxin in an animal model. But the underlying mechanism has not been fully elucidated.

Methods

SD male rats in adult were used to model HS and treated with DHFZT. The serum concentration of endotoxin were analyzed by dynamic turbidimetric method. In addition, the blood flow of small intestine were measured using laser speckle technique. Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP) and zonula occludens (ZO)-1 protein, intestinal fatty acid binding protein (IFABP) were measured by Western Blotting, RT-PCR, ELISA respectively.

Results

Present study showed that DHFZT markedly elevated the blood flow of small intestine, protected the intestinal barrier function by up-regulating the expression of ZO-1 protein and down-regulating expression of p-VASP, and notely decreased serum concentration of IFABP and endotoxin in HS.

Conclusions

These results reveal that DHFZT improves intestinal blood flow, protects the intestinal barrier function, and ameliorates intestinal endotoxaemia in rats with HS.

【 授权许可】

   
2014 Lu et al.; licensee BioMed Central Ltd.

【 图 表 】

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