Central-stimulating and analgesic activity of the ethanolic extract of Alternanthera sessilis in mice

BMC Complementary and Alternative Medicine

Research Article

Ismet Ara Jahan¹  Hemayet Hossain¹  Khalijah Awang²  Abdulwali Ablat³  Samir K Sadhu⁴  Shaikh J Uddin⁴  Himangsu Mondal⁴  Md Golam Hossain⁴  Jamil A Shilpi⁵  Md Khirul Islam⁶  Sanjib Saha⁶ 

[1] BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), 1205, Dhaka, Bangladesh;Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;Institute of Biological Science, University of Malaya, 50603, Kuala Lumpur, Malaysia;Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Khulna University, 9208, Khulna, Bangladesh;Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Khulna University, 9208, Khulna, Bangladesh;Centre for Natural Products and Drug Discovery, University of Malaya, 50603, Kuala Lumpur, Malaysia;Phytochemistry and Pharmacology Research Laboratory, Pharmacy Discipline, Khulna University, 9208, Khulna, Bangladesh;Department of Biochemistry, Faculty of Mathematics and Natural Sciences, University of Turku, FI-20500, Turku, Finland;

关键词: Alternanthera sessilis;    Central stimulating activity;    Pentobarbitone induced sleeping time;    Acetic acid induced writhing test;   

DOI  :  10.1186/1472-6882-14-398

 received in 2014-06-15, accepted in 2014-10-08,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAlternanthera sessilis is a popular vegetable and used in traditional medicinal practice of Bangladesh and other parts of Asia to relive tiredness, laziness, and sleeps as well as pain and inflammation. However, no report was found on the neuropharmacological and analgesic activity of this plant to-date. Present study was undertaken to evaluate the neuropharmacological and analgesic activity of the ethanol extract of A. sessilis whole plant (ETAS) in mice models.MethodsCentral stimulating activity was investigated by pentobarbitone induced sleeping time, open field, and hole cross tests. Analgesic activity was evaluated by acetic acid induced writhing and hot-plate methods. The tests were performed at 250 and 500 mg/kg body weight dose levels.ResultsIn sleeping time test, ETAS significantly (p < 0.001) increased the onset of sleep, and decreased the duration of sleep. In open field and hole cross tests, ETAS significantly (p < 0.001) increased the movements of mice which persisted throughout the study period. In writhing test, ETAS showed, significant (p < 0.001) inhibition of writhing reflex. In hot plate test, ETAS significantly (p < 0.001) raised the pain threshold. In HPLC analysis for polyphenols, (+)-catechin, rutin, ellagic acid, and quercetin were detected in ETAS (117.72, 490.74, 3007.26, and 13.85 mg/100 g of dry extract, respectively).ConclusionPresent study supported the traditional uses of A. sessilis and indicated that the plant can be a potential source of bioactive molecules.

【 授权许可】

Unknown   
© Mondal et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.