KYNA analogue SZR72 modifies CFA-induced dural inflammation- regarding expression of pERK1/2 and IL-1β in the rat trigeminal ganglion

The Journal of Headache and Pain

Short Report

L. S. Kruse¹  L. Edvinsson²  K. Warfvinge³  M. Lukács⁴  J. Tajti⁵  L. Vécsei⁶  J. Toldi⁷  F. Fülöp⁸ 

[1] Department of Clinical Experimental Research, Copenhagen University, Glostrup Hospital, Copenhagen, Denmark;Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, Lund, Sweden;Department of Clinical Experimental Research, Copenhagen University, Glostrup Hospital, Copenhagen, Denmark;Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, Lund, Sweden;Department of Clinical Experimental Research, Copenhagen University, Glostrup Hospital, Copenhagen, Denmark;Department of Medicine, Institute of Clinical Sciences, Lund University, Sölvegatan 17, SE 221 84, Lund, Sweden;Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, Lund, Sweden;Department of Neurology, University of Szeged, Szeged, Hungary;Department of Neurology, University of Szeged, Szeged, Hungary;Department of Neurology, University of Szeged, Szeged, Hungary;MTA SZTE Neuroscience Research Group, Szeged, Hungary;Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary;MTA SZTE Neuroscience Research Group, Szeged, Hungary;Institute of Pharmaceutical Chemistry and MTA-SZTE Research Group for Stereochemistry, University of Szeged, Szeged, Hungary;

关键词: Complete Freund’s Adjuvant;    Dura mater;    Trigeminal ganglion;    pERK1/2;    IL-1β;    KYNA;   

DOI  :  10.1186/s10194-016-0654-5

 received in 2016-05-21, accepted in 2016-06-21,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundNeurogenic inflammation has for decades been considered an important part of migraine pathophysiology. In the present study, we asked the question if administration of a novel kynurenic acid analogue (SZR72), precursor of an excitotoxin antagonist and anti-inflammatory substance, can modify the neurogenic inflammatory response in the trigeminal ganglion.MethodsInflammation in the trigeminal ganglion was induced by local dural application of Complete Freunds Adjuvant (CFA). Levels of phosphorylated MAP kinase pERK1/2 and IL-1β expression in V1 region of the trigeminal ganglion were investigated using immunohistochemistry and Western blot.FindingsPretreatment with one dose of SZR72 abolished the CFA-induced pERK1/2 and IL-1β activation in the trigeminal ganglion. No significant change was noted in case of repeated treatment with SZR72 as compared to a single dose.ConclusionsThis is the first study that demonstrates that one dose of KYNA analog before application of CFA can give anti-inflammatory response in a model of trigeminal activation, opening a new line for further investigations regarding possible effects of KYNA derivates.

【 授权许可】

CC BY   
© The Author(s). 2016