Standardization of Antigen-Emulsion Preparations for the Induction of Autoimmune Disease Models

Frontiers in Immunology

Felix I. Clanchy¹  B. Thomas Bäckström²  Hans Bolinsson³  Richard O. Williams⁴  Louise M. Topping⁴  Laura Romero-Castillo⁵  Rikard Holmdahl⁵  Vilma Urbonaviciute⁵ 

[1] Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, United Kingdom;Department of Autoimmunity, BTB Emulsions AB, Malmo, Sweden;Department of Food Technology, Engineering and Nutrition, Lund University, Lund, Sweden;Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, United Kingdom;Medical Inflammation Research, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden;The Rausing Laboratory, Division of Neurosurgery, Department of Clinical Sciences, Lund University, Lund, Sweden;

关键词: autoimmunity;    animal disease models;    EAE;    CIA;    antigen emulsions;    Complete Freund’s Adjuvant;   

DOI  :  10.3389/fimmu.2022.892251

来源: DOAJ

【 摘 要 】

Autoimmune murine disease models are vital tools for identifying novel targets and finding better treatments for human diseases. Complete Freund’s adjuvant is commonly used to induce disease in autoimmune models, and the quality of the adjuvant/autoantigen emulsion is of critical importance in determining reproducibility. We have established an emulsification method using a standard homogenizer and specially designed receptacle. Emulsions are easy to prepare, form stable and uniform water-in-oil particles, are faster to make than the traditional syringe method, use less material and are designed to fill syringes with ease. In the present study, we have validated the emulsions for induction of experimental autoimmune encephalitis, collagen II induced arthritis, antigen induced arthritis, and delayed type hypersensitivity models. These models were induced consistently and reproducibly and, in some cases, the new method outperformed the traditional method. The method described herein is simple, cost-effective and will reduce variability, thereby requiring fewer animals for in vivo research involving animal models of autoimmune disease and in vaccine development.

【 授权许可】

Unknown