Two jasmonic acid carboxyl methyltransferases in Gossypium hirsutum involved in MeJA biosynthesis may contribute to plant defense

Frontiers in Plant Science

Plant Science

Xinzheng Huang¹  Khalid Hussain Dhiloo²  Yongjun Zhang³  Beibei Lv³  Xiaohe Liu³  Weixia Jing⁴  Junfeng Kou⁵  Dong Teng⁶  Danyang Zhao⁷ 

[1] College of Plant Protection, China Agricultural University, Beijing, China;Department of Entomology, Faculty of Crop Protection, Sindh Agriculture University, Tandojam, Pakistan;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;College of Plant Protection, Shandong Agricultural University, Taian, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;Institute of Plant Protection, Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, China;

关键词: phytohormone;    jasmonic acid carboxyl methyltransferases;    biosynthesis;    HIPVs;    plant defense;   

DOI  :  10.3389/fpls.2023.1249226

 received in 2023-06-28, accepted in 2023-08-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Jasmonic acid (JA) and methyl jasmonate (MeJA), the crucial plant hormones, can induce the emission of plant volatiles and regulate the behavioral responses of insect pests or their natural enemies. In this study, two jasmonic acid carboxyl methyltransferases (JMTs), GhJMT1 and GhJMT2, involved in MeJA biosynthesis in Gossypium. hirsutum were identified and further functionally confirmed. In vitro, recombinant GhJMT1 and GhJMT2 were both responsible for the conversion of JA to MeJA. Quantitative real-time PCR (qPCR) measurement indicated that GhJMT1 and GhJMT2 were obviously up-regulated in leaves and stems of G. hirsutum after being treated with MeJA. In gas chromatography-mass spectrometry (GC-MS) analysis, MeJA treatment significantly induced plant volatiles emission such as (E)-β-ocimene, (Z)-3-hexenyl acetate, linalool and (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), which play vital roles in direct and indirect plant defenses. Moreover, antennae of parasitoid wasps Microplitis mediator showed electrophysiological responses to MeJA, β-ocimene, (Z)-3-hexenyl acetate and linalool at a dose dependent manner, while our previous research revealed that DMNT excites electrophysiological responses and behavioral tendencies. These findings provide a better understanding of MeJA biosynthesis and defense regulation in upland cotton, which lay a foundation to JA and MeJA employment in agricultural pest control.

【 授权许可】

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Copyright © 2023 Teng, Jing, Lv, Huang, Zhao, Kou, Liu, Dhiloo and Zhang