Structure modification of an antibiotic: by engineering the fusaricidin bio-synthetase A in Paenibacillus polymyxa

Frontiers in Microbiology

Microbiology

Yunlong Li¹  Sanfeng Chen² 

[1] Chengdu NewSun Crop Science Co. Ltd., Chengdu, China;State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China;

关键词: gene engineering;    [ΔAla] fusaricidin LI-F07a;    structure modification;    homologous recombination;    biocontrol agent;   

DOI  :  10.3389/fmicb.2023.1239958

 received in 2023-06-14, accepted in 2023-09-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

Fusaricidin, a lipopeptide antibiotic, is specifically produced by Paenibacillus polymyxa strains, which could strongly inhibit Fusarium species fungi. Fusaricidin bio-synthetase A (FusA) is composed of six modules and is essential for synthesizing the peptide moiety of fusaricidin. In this study, we confirmed the FusA of Paenibacillus polymyxa strain WLY78 involved in producing Fusaricidin LI-F07a. We constructed six engineered strains by deletion of each module within FusA from the genome of strain WLY78. One of the engineered strains is able to produce a novel compound that exhibits better antifungal activity than that of fusaricidin LI-F07a. This new compound, known as fusaricidin [ΔAla6] LI-F07a, has a molecular weight of 858. Our findings reveal that it exhibits a remarkable 1-fold increase in antifungal activity compared to previous fusaricidin, and the fermentation yield reaches ~55 mg/L. This research holds promising implications for plant protection against infections caused by Fusarium and Botrytis pathogen infection.

【 授权许可】

Unknown   
Copyright © 2023 Li and Chen.