Cloning and expression of visfatin and screening of oligopeptides binding with visfatin

International Journal of Clinical and Experimental Medicine

De Min Yu¹  Lin Ge¹  Xin Yu Liu¹ 

关键词: Visfatin;    adipokine;    gene engineering;    fusion expression;    phage display;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Purpose: In the present study, we constructed an efficient expression system for visfatin and identified the peptide sequences which binding to visfatin. Methods and results: The fusion protein was identified by SDS-PAGE and Western blot. The yield of visfatin was 300 mg/L culture medium with optimal conditions. The recombinant visfatin binds with insulin receptor with a dose-dependent effect. All of the 24 sequence identified by ELISA were able to bind to visfatin specifically. Among the 24 DNA sequences, there were 8 clones of AAKTPTE, 4 clones of ATTVPAS and 4 clones of MSLQQEH. Conclusion: The sequence of AA(X)TPT(X) was the most frequently existed sequence in all of sequences analyzed, which suggests that AA(X)TPT(X) is likely to be the essential motif in peptides which visfatin could bind with.

【 授权许可】

Unknown