Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

S. O. Peters¹  D. R. Bresnahan¹  K. J. Fresa²  E. M. Carnevale²  G. D. Catandi²  L. J. Maclellan²  C. D. Broeckling³ 

[1] Department of Animal Sciences, Berry College, Mount Berry, GA, United States;Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, United States;Proteomic and Metabolomics Core Facility, Colorado State University, Fort Collins, CO, United States;

关键词: mare;    oocyte;    age;    maturation;    cumulus;    granulosa;    follicle;    metabolome;   

DOI  :  10.3389/fcell.2023.1239154

 received in 2023-06-12, accepted in 2023-08-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Oocyte quality and fertility decline with advanced maternal age. During maturation within the ovarian follicle, the oocyte relies on the associated somatic cells, specifically cumulus and granulosa cells, to acquire essential components for developmental capacity.Methods: A nontargeted metabolomics approach was used to investigate the effects of mare age on different cell types within the dominant, follicular-phase follicle at three time points during maturation. Metabolomic analyses from single oocytes and associated cumulus and granulosa cells allowed correlations of metabolite abundance among cell types.Results and Discussion: Overall, many of the age-related changes in metabolite abundance point to Impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. Supporting findings include a higher abundance of glutamic acid and triglycerides and lower abundance of ceramides in oocytes and somatic follicular cells from old than young mares. Lower abundance of alanine in all follicular cell types from old mares, suggests limited anaerobic energy metabolism. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.

【 授权许可】

Unknown   
Copyright © 2023 Catandi, Bresnahan, Peters, Fresa, Maclellan, Broeckling and Carnevale.