Molecular characterization and differential effects of levofloxacin and ciprofloxacin on the potential for developing quinolone resistance among clinical Pseudomonas aeruginosa isolates

Frontiers in Microbiology

Microbiology

Bassam El Hafi¹  George F. Araj²  Ghassan M. Matar³  Ahmad Sleiman³  Antoine G. Abou Fayad³  Jim Abi Frem⁴  George Doumat⁴  Amal Gharamti⁴  Souha S. Kanj⁵  Zeina A. Kanafani⁵  Michel Doumith⁶  Majed F. AlGhoribi⁶ 

[1] Center for Infectious Diseases Research, American University of Beirut, Beirut, Lebanon;Center for Infectious Diseases Research, American University of Beirut, Beirut, Lebanon;Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Beirut, Lebanon;Center for Infectious Diseases Research, American University of Beirut, Beirut, Lebanon;Faculty of Medicine, Department of Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut, Lebanon;World Health Organization Collaborating Center for Reference and Research on Bacterial Pathogens, Beirut, Lebanon;Division of Infectious Diseases, Department of Internal Medicine, American University of Beirut Medical Center, Beirut, Lebanon;Division of Infectious Diseases, Department of Internal Medicine, American University of Beirut Medical Center, Beirut, Lebanon;Center for Infectious Diseases Research, American University of Beirut, Beirut, Lebanon;Infectious Diseases Research Department, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia;King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia;

关键词: Pseudomonas aeruginosa;    hospital-acquired infection;    nosocomial infection;    antimicrobial resistance;    fluoroquinolone resistance;    fitness cost;   

DOI  :  10.3389/fmicb.2023.1209224

 received in 2023-04-20, accepted in 2023-08-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundFluoroquinolones are some of the most used antimicrobial agents for the treatment of Pseudomonas aeruginosa. This study aimed at exploring the differential activity of ciprofloxacin and levofloxacin on the selection of resistance among P. aeruginosa isolates at our medical center.Methods233 P. aeruginosa clinical isolates were included in this study. Antimicrobial susceptibility testing (AST) was done using disk diffusion and broth microdilution assays. Random Amplification of Polymorphic DNA (RAPD) was done to determine the genetic relatedness between the isolates. Induction of resistance against ciprofloxacin and levofloxacin was done on 19 isolates. Fitness cost assay was done on the 38 induced mutants and their parental isolates. Finally, whole genome sequencing was done on 16 induced mutants and their 8 parental isolates.ResultsAST results showed that aztreonam had the highest non-susceptibility. RAPD results identified 18 clusters. The 19 P. aeruginosa isolates that were induced against ciprofloxacin and levofloxacin yielded MICs ranging between 16 and 256 μg/mL. Levofloxacin required fewer passages in 10 isolates and the same number of passages in 9 isolates as compared to ciprofloxacin to reach their breakpoints. Fitness cost results showed that 12 and 10 induced mutants against ciprofloxacin and levofloxacin, respectively, had higher fitness cost when compared to their parental isolates. Whole genome sequencing results showed that resistance to ciprofloxacin and levofloxacin in sequenced mutants were mainly associated with alterations in gyrA, gyrB and parC genes.ConclusionUnderstanding resistance patterns and risk factors associated with infections is crucial to decrease the emerging threat of antimicrobial resistance.

【 授权许可】

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Copyright © 2023 Kanafani, Sleiman, Frem, Doumat, Gharamti, El Hafi, Doumith, AlGhoribi, Kanj, Araj, Matar and Abou Fayad.