Elucidating the molecular mechanisms of ozone therapy for neuropathic pain management by integrated transcriptomic and metabolomic approach

Frontiers in Genetics

Genetics

Xiaolan Yang¹  Yong Wang¹  Keyang Wang¹  Min Chen¹  Chaoming Chen¹  Zhengping Chen¹  Shu Ou¹  Wang Zhao² 

[1] Department of Neurology, The Fengjie People’s Hospital, Fengjie Branch of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China;Department of Neurology, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China;

关键词: ozone therapy;    neuropathic pain;    transcriptomics;    metabolomics;    mechanism of action;    multi-omics;   

DOI  :  10.3389/fgene.2023.1231682

 received in 2023-05-30, accepted in 2023-08-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Neuropathic pain remains a prevalent and challenging condition to treat, with current therapies often providing inadequate relief. Ozone therapy has emerged as a promising treatment option; however, its mechanisms of action in neuropathic pain remain poorly understood.Methods: In this study, we investigated the effects of ozone treatment on gene expression and metabolite levels in the brainstem and hypothalamus of a rat model, using a combined transcriptomic and metabolomic approach.Results: Our findings revealed significant alterations in key genes, including DCST1 and AIF1L, and metabolites such as Aconitic acid, L-Glutamic acid, UDP-glucose, and Tyrosine. These changes suggest a complex interplay of molecular pathways and region-specific mechanisms underlying the analgesic effects of ozone therapy.Discussion: Our study provides insights into the molecular targets of ozone treatment for neuropathic pain, laying the groundwork for future research on validating these targets and developing novel therapeutic strategies.

【 授权许可】

Unknown   
Copyright © 2023 Yang, Chen, Wang, Chen, Wang, Chen, Zhao and Ou.