The prevalence, genetic diversity and evolutionary analysis of cachavirus firstly detected in northeastern China

Frontiers in Veterinary Science

Veterinary Science

Xin Yan¹  Zhiyuan Guo²  Zaixing Yang²  Yue Bai²  Kongrui Xiang²  Haikun Shangguan²  Junwei Ge³  Lili Zhao⁴  Nuowa Li⁵ 

[1] China Animal Health and Epidemiology Center, Qingdao, China;College of Veterinary Medicine, Northeast Agricultural University, Harbin, China;College of Veterinary Medicine, Northeast Agricultural University, Harbin, China;Heilongjiang Provincial Key Laboratory of Zoonosis, Harbin, China;State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China;State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China;College of Veterinary Medicine, Northeast Agricultural University, Harbin, China;

关键词: cachavirus;    chapparvovirus;    phylogenetic analysis;    evolution;    codon usage;   

DOI  :  10.3389/fvets.2023.1233972

 received in 2023-06-08, accepted in 2023-08-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

Canine cachavirus is a novel parvovirus belonging to the genus Chaphamaparvovirus that was first detected in dogs in the United States. However, our knowledge of the prevalence and genetic characteristics of cachavirus is relatively limited. In this study, 325 canine fecal specimens collected from healthy and diarrheic dogs in northeastern China were screened with PCR. Twenty-two of the 325 (6.8%) samples were positive for cachavirus. The diarrhea samples showed high viral coinfection rates, and we detected coinfections with canine astrovirus (CaAstV) and cachavirus for the first time. A sequence analysis revealed that the Chinese cachavirus strains have point mutations in four consecutive amino acid codons relative to the original American strain. A codon usage analysis of the VP1 gene showed that most preferred codons in cachavirus were A- or T-ending codons, as in traditional canine parvovirus 2. A co-evolutionary analysis showed that cachavirus has undergone cospeciation with its hosts and has been transmitted among different host species. Our findings extend the limited cachavirus sequences available, and provide detailed molecular characterization of the strains in northeastern China. Further epidemiological surveillance is required to determine the significance and evolution of cachavirus.

【 授权许可】

Unknown   
Copyright © 2023 Li, Bai, Yan, Guo, Xiang, Yang, Shangguan, Ge and Zhao.