Analysis of the complete chloroplast genomes of Scutellaria tsinyunensis and Scutellaria tuberifera (Lamiaceae)

Mitochondrial DNA Part B

Yuanyu Shan¹  Jingling Li¹  Siyuan Zeng¹  Shunyuan Yong¹  Xiaoying Pei¹  Qiulin Qin¹  Jie Yu² 

[1] College of Horticulture and Landscape Architecture, Southwest University, Chongqing, PR Chin;College of Horticulture and Landscape Architecture, Southwest University, Chongqing, PR Chin;Ministry of Education, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Chongqing, PR Chin;

关键词: Scutellaria;    chloroplast genome;    evolution;    hypervariable regions;    phylogenetic analysis;   

DOI  :  10.1080/23802359.2021.1920491

来源: Taylor & Francis

【 摘 要 】

Scutellaria Linn. is a perennial herb with about 300 species. This genus has high medicinal value and many are used in Traditional Chinese Medicine (TCM). In this study, we sequenced and assembled the complete chloroplast genomes of Scutellaria tsinyunensis and S. tuberifera. Subsequently, we conducted a comprehensive comparative genomics analysis with 12 other published Scutellaria species. These genomes all had a conserved quartile structure, and the gene contents, gene sequences and GC contents are highly similar. The study on the genetic characteristics and nucleotide substitution rate of different genes found that the protein-coding genes of chloroplasts have differed greatly. Most genes are under purifying selection, but the rps12 gene may have undergone positive selection. Besides, we identified three hypervariable regions as potential markers for Scutellaria taxa, which could play an important role in species identification of Scutellaria. Phylogenetic analysis showed that the 14 Scutellaria taxa were divided into two major clades. Moreover, the variation of IR regions is closely related to the evolutionary history as was reconstructed based on SNPs. In conclusion, we provided two high-quality chloroplast reference genomes of Scutellaria, this reliable information and genomic resources are valuable for developing of efficient DNA barcodes as reconstruction of chloroplast evolutionary history of the genus.

【 授权许可】

CC BY