Genome-wide characterization of the soybean DOMAIN OF UNKNOWN FUNCTION 679 membrane protein gene family highlights their potential involvement in growth and stress response

Frontiers in Plant Science

Plant Science

Jin Hoon Jang¹  Jae Hyun Lee¹  Ok Ran Lee¹  Bhagwat Nawade¹  Tejas C. Bosamia² 

[1] Department of Applied Plant Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea;Interdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju, Republic of Korea;Plant Omics Division, Council of Scientific and Industrial Research-Central Salt and Marine Chemical Research Institute (CSIR-CSMCRI), Bhavnagar, Gujarat, India;

关键词: DUF679;    soybean;    abiotic stress;    genomic analysis;    cis-acting elements;   

DOI  :  10.3389/fpls.2023.1216082

 received in 2023-05-03, accepted in 2023-08-22,  发布年份 2023

来源: Frontiers

【 摘 要 】

The DMP (DUF679 membrane proteins) family is a plant-specific gene family that encodes membrane proteins. The DMP family genes are suggested to be involved in various programmed cell death processes and gamete fusion during double fertilization in Arabidopsis. However, their functional relevance in other crops remains unknown. This study identified 14 genes from the DMP family in soybean (Glycine max) and characterized their physiochemical properties, subcellular location, gene structure, and promoter regions using bioinformatics tools. Additionally, their tissue-specific and stress-responsive expressions were analyzed using publicly available transcriptome data. Phylogenetic analysis of 198 DMPs from monocots and dicots revealed six clades, with clade-I encoding senescence-related AtDMP1/2 orthologues and clade-II including pollen-specific AtDMP8/9 orthologues. The largest clade, clade-III, predominantly included monocot DMPs, while monocot- and dicot-specific DMPs were assembled in clade-IV and clade-VI, respectively. Evolutionary analysis suggests that soybean GmDMPs underwent purifying selection during evolution. Using 68 transcriptome datasets, expression profiling revealed expression in diverse tissues and distinct responses to abiotic and biotic stresses. The genes Glyma.09G237500 and Glyma.18G098300 showed pistil-abundant expression by qPCR, suggesting they could be potential targets for female organ-mediated haploid induction. Furthermore, cis-acting regulatory elements primarily related to stress-, hormone-, and light-induced pathways regulate GmDMPs, which is consistent with their divergent expression and suggests involvement in growth and stress responses. Overall, our study provides a comprehensive report on the soybean GmDMP family and a framework for further biological functional analysis of DMP genes in soybean or other crops.

【 授权许可】

Unknown   
Copyright © 2023 Nawade, Bosamia, Lee, Jang and Lee