Comprehensive Genomic Analysis and Expression Profiling of Diacylglycerol Kinase (DGK) Gene Family in Soybean (Glycine max) under Abiotic Stresses

International Journal of Molecular Sciences

卷:20

Toi Ketehouli¹  Fa-Wei Wang¹  Hai-Yan Li¹  KueFoka Idrice Carther¹  Nan Ye¹  Yuan-Yuan Dong¹  Xiao-Wei Li¹  Na Yao¹  Xiu-Ming Liu¹  Wei-Can Liu¹  Yan-Hai Yang¹  Nan Wang¹ 

[1] College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China;

关键词: soybean;    diacylglycerol kinase;    phosphatidic acid (PA);    abiotic stress;    gene family lipid signaling;   

DOI  :  10.3390/ijms20061361

来源: DOAJ

【 摘 要 】

Diacylglycerol kinase (DGK) is an enzyme that plays a pivotal role in abiotic and biotic stress responses in plants by transforming the diacylglycerol into phosphatidic acid. However, there is no report on the characterization of soybean DGK genes in spite of the availability of the soybean genome sequence. In this study, we performed genome-wide analysis and expression profiling of the DGK gene family in the soybean genome. We identified 12 DGK genes (namely GmDGK1-12) which all contained conserved catalytic domains with protein lengths and molecular weights ranging from 436 to 727 amino acids (aa) and 48.62 to 80.93 kDa, respectively. Phylogenetic analyses grouped GmDGK genes into three clusters—cluster I, cluster II, and cluster III—which had three, four, and five genes, respectively. The qRT-PCR analysis revealed significant GmDGK gene expression levels in both leaves and roots coping with polyethylene glycol (PEG), salt, alkali, and salt/alkali treatments. This work provides the first characterization of the DGK gene family in soybean and suggests their importance in soybean response to abiotic stress. These results can serve as a guide for future studies on the understanding and functional characterization of this gene family.

【 授权许可】

Unknown