Comprehensive analyses of the citrus WRKY gene family involved in the metabolism of fruit sugars and organic acids

Frontiers in Plant Science

Plant Science

Ling Liao¹  Bo Xiong¹  Xinxia Yang¹  Chunmei Yin¹  Zhihui Wang¹  Xingyu Lin¹  Kexin Liu¹  Qin Li¹  Guochao Sun¹  Siya He¹  Jiaxian He¹  Mingfei Zhang¹  Wen Lu¹  Xun Wang¹ 

[1] College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China;

关键词: WRKY gene family;    citrus;    fruit quality;    sugar;    organic acid;    expression network;   

DOI  :  10.3389/fpls.2023.1264283

 received in 2023-07-20, accepted in 2023-08-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Sugars and organic acids are the main factors determining the flavor of citrus fruit. The WRKY transcription factor family plays a vital role in plant growth and development. However, there are still few studies about the regulation of citrus WRKY transcription factors (CsWRKYs) on sugars and organic acids in citrus fruit. In this work, a genome-wide analysis of CsWRKYs was carried out in the citrus genome, and a total of 81 CsWRKYs were identified, which contained conserved WRKY motifs. Cis-regulatory element analysis revealed that most of the CsWRKY promoters contained several kinds of hormone-responsive and abiotic-responsive cis-elements. Furthermore, gene expression analysis and fruit quality determination showed that multiple CsWRKYs were closely linked to fruit sugars and organic acids with the development of citrus fruit. Notably, transcriptome co-expression network analysis further indicated that three CsWRKYs, namely, CsWRKY3, CsWRKY47, and CsWRKY46, co-expressed with multiple genes involved in various pathways, such as Pyruvate metabolism and Citrate cycle. These CsWRKYs may participate in the metabolism of fruit sugars and organic acids by regulating carbohydrate metabolism genes in citrus fruit. These findings provide comprehensive knowledge of the CsWRKY family on the regulation of fruit quality.

【 授权许可】

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Copyright © 2023 Zhang, Lu, Yang, Li, Lin, Liu, Yin, Xiong, Liao, Sun, He, He, Wang and Wang