Transcriptomic Analysis of Late-Ripening Sweet Orange Fruits (Citrus sinensis) after Foliar Application of Glomalin-Related Soil Proteins

Agriculture

Abeer Hashem¹  Hui-Hui Wu²  Ying-Ning Zou²  Qiang-Sheng Wu²  Anoop Kumar Srivastava³  Elsayed Fathi Abd_Allah⁴  Yan Li⁵ 

[1] Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia;College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China;ICAR-Central Citrus Research Institute, Nagpur 440033, India;Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia;Xingshan County Special Products Bureau, Xingshan 443711, China;

关键词: citrus;    fruit quality;    gene expression;    glomalin;    hexose;    mycorrhiza;   

DOI  :  10.3390/agriculture11111171

来源: DOAJ

【 摘 要 】

Glomalin, one of the glycoproteins generated in the spores and hyphae of arbuscular mycorrhizal (AM) fungi, has multiple functions in plants and soil, while the role of foliar spray of easily extractable glomalin-related soil proteins (EE-GRSP) in citrus fruits is not well defined. Our study aimed to use referenced transcriptome sequencing to uncover the mechanism and the role of exogenous EE-GRSP in two late-ripening varieties of sweet orange (Citrus sinensis) fruits including Navel Lane Late (LW) and Rohde Red Valencia (XC). The 1804 and 1861 differentially expressed genes were identified in fruits of LW and XC, respectively, following foliar spray of EE-GRSP. Photosynthesis ranked second in the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolism in the LW variety, and carbon fixation in photosynthetic organizations ranked first in KEGG metabolism in the XC variety. The response to foliar spray of EE-GRSP affected the fruit starch and sucrose metabolism of KEGG, with 15 (10 up-regulated and 5 down-regulated) and 13 (2 up-regulated and 11 down-regulated) differentially expressed genes identified in the LW and XC variety, respectively. Cs5g19060 (sucrose phosphate synthase 4) was activated and reduced by EE-GRSP on XC and LW, respectively. Cs1g18220 (β-fructofuranosidase) and Cs2g12180 (glycosyl hydrolase family 9) genes were up-regulated and down-regulated in LW and XC, respectively. These results established the involvement of molecular signaling in response to foliar spray of EE-GRSP activating fruit sugar metabolism is dependent on citrus varieties.

【 授权许可】

Unknown