A plant virus protein, NIa-pro, interacts with Indole-3-acetic acid-amido synthetase, whose levels positively correlate with disease severity

Frontiers in Plant Science

Plant Science

Hira Kamal¹  Prabu Gnanasekaran¹  Ying Zhai¹  Hanu R. Pappu¹  Andrei Smertenko² 

[1] Department of Plant Pathology, Washington State University, Pullman, WA, United States;Institute of Biological Chemistry, Washington State University, Pullman, WA, United States;

关键词: potato virus Y;    auxin conjugation;    indole-3-acetic acid-amido synthetase;    auxin homeostasis;    plant innate immunity;   

DOI  :  10.3389/fpls.2023.1112821

 received in 2022-11-30, accepted in 2023-08-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

Potato virus Y (PVY) is an economically important plant pathogen that reduces the productivity of several host plants. To develop PVY-resistant cultivars, it is essential to identify the plant-PVY interactome and decipher the biological significance of those molecular interactions. We performed a yeast two-hybrid (Y2H) screen of Nicotiana benthamiana cDNA library using PVY-encoded NIa-pro as the bait. The N. benthamiana Indole-3-acetic acid-amido synthetase (IAAS) was identified as an interactor of NIa-pro protein. The interaction was confirmed via targeted Y2H and bimolecular fluorescence complementation (BiFC) assays. NIa-pro interacts with IAAS protein and consequently increasing the stability of IAAS protein. Also, the subcellular localization of both NIa-pro and IAAS protein in the nucleus and cytosol was demonstrated. By converting free IAA (active form) to conjugated IAA (inactive form), IAAS plays a crucial regulatory role in auxin signaling. Transient silencing of IAAS in N. benthamiana plants reduced the PVY-mediated symptom induction and virus accumulation. Conversely, overexpression of IAAS enhanced symptom induction and virus accumulation in infected plants. In addition, the expression of auxin-responsive genes was found to be downregulated during PVY infection. Our findings demonstrate that PVY NIa-pro protein potentially promotes disease development via modulating auxin homeostasis.

【 授权许可】

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Copyright © 2023 Gnanasekaran, Zhai, Kamal, Smertenko and Pappu