MAG2 and MAL Regulate Vesicle Trafficking and Auxin Homeostasis With Functional Redundancy

Frontiers in Plant Science

Zhengbiao Long¹  Xiaonan Zhao¹  Lixi Jiang¹  Shanwen Sun²  Ying Li²  Yuqi Liu²  Xiaomeng Zhang²  Hailong Zhang²  Xiaohui Ma²  Yiming Zhang²  Lixin Li²  Jian-Kang Zhu³  Rongxia Li³  Li Tan³ 

[1] Institute of Crop Science, Zhejiang University, Hangzhou, China;Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, College of Life Sciences, Ministry of Education, Northeast Forestry University, Harbin, China;Shanghai Center for Plant Stress Biology, Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China;

关键词: MAG2 and MAL;    vesicle trafficking;    auxin homeostasis;    plant development and stress response;    proteomic analysis;   

DOI  :  10.3389/fpls.2022.849532

来源: DOAJ

【 摘 要 】

Auxin is a central phytohormone and controls almost all aspects of plant development and stress response. Auxin homeostasis is coordinately regulated by biosynthesis, catabolism, transport, conjugation, and deposition. Endoplasmic reticulum (ER)-localized MAIGO2 (MAG2) complex mediates tethering of arriving vesicles to the ER membrane, and it is crucial for ER export trafficking. Despite important regulatory roles of MAG2 in vesicle trafficking, the mag2 mutant had mild developmental abnormalities. MAG2 has one homolog protein, MAG2-Like (MAL), and the mal-1 mutant also had slight developmental phenotypes. In order to investigate MAG2 and MAL regulatory function in plant development, we generated the mag2-1 mal-1 double mutant. As expected, the double mutant exhibited serious developmental defects and more alteration in stress response compared with single mutants and wild type. Proteomic analysis revealed that signaling, metabolism, and stress response in mag2-1 mal-1 were affected, especially membrane trafficking and auxin biosynthesis, signaling, and transport. Biochemical and cell biological analysis indicated that the mag2-1 mal-1 double mutant had more serious defects in vesicle transport than the mag2-1 and mal-1 single mutants. The auxin distribution and abundance of auxin transporters were altered significantly in the mag2-1 and mal-1 single mutants and mag2-1 mal-1 double mutant. Our findings suggest that MAG2 and MAL regulate plant development and auxin homeostasis by controlling membrane trafficking, with functional redundancy.

【 授权许可】

Unknown