Lack of associations of microRNAs with severe NAFLD in people living with HIV: discovery case-control study

Frontiers in Endocrinology

Endocrinology

Jose M. Moyano¹  Inmaculada Ruiz-Cáceres²  Marı́a Casares-Jiménez²  Diana Corona-Mata²  Antonio Rivero-Juárez³  Antonio Rivero³  Angela Camacho-Espejo³  Pedro López-López³  Ignacio Pérez-Valero³  Javier Caballero-Gómez⁴  Mario Frías⁴ 

[1] Department of Computer Science and Artificial Intelligence, Andalusian Research Institute in Data Science and Computational Intelligence (DaSCI), University of Granada, Granada, Spain;Unit of Infectious Diseases, Hospital Universitario Reina Sofia, Clinical Virology and Zoonoses, Maimonides Biomedical Research Institute of Cordoba, University of Córdoba, Córdoba, Spain;Unit of Infectious Diseases, Hospital Universitario Reina Sofia, Clinical Virology and Zoonoses, Maimonides Biomedical Research Institute of Cordoba, University of Córdoba, Córdoba, Spain;CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC, ISCIII), Madrid, Spain;Unit of Infectious Diseases, Hospital Universitario Reina Sofia, Clinical Virology and Zoonoses, Maimonides Biomedical Research Institute of Cordoba, University of Córdoba, Córdoba, Spain;CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC, ISCIII), Madrid, Spain;Animal Health Department, Animal Health and Zoonoses Research Group (GISAZ), University of Córdoba, Córdoba, Spain;

关键词: microRNA;    HIV;    NAFLD;    CAP;    PLWH;    steatosis;   

DOI  :  10.3389/fendo.2023.1230046

 received in 2023-05-27, accepted in 2023-08-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Background & objectiveNonalcoholic fatty liver disease (NAFLD) is highly prevalent in people living with HIV (PLWH) and the expression of some microRNAs could be useful as biomarkers for the diagnosis of NAFLD. The aim of this study was to identify patterns of differential expression of microRNAs in PLWH and assess their diagnostic value for NALFD.MethodsA discovery case-control study with PLWH was carried out. The expression of miRNAs was determined using HTG EdgeSeq technology. Cases were defined as patients with severe NAFLD and controls as patients without NAFLD, characterized using the controlled attenuation parameter (CAP). Cases and controls were matched 1:1 for age, sex, BMI, CD4+ lymphocyte count, active HCV infection, and ART regimen.ResultsSerum 2,083 simultaneous microRNA transcripts were analyzed using HTG technology and compared between cases and controls. Forty-five patients, 23 cases, and 22 controls were included in the study. In the analysis of the expression pattern of the 2,083 microRNAs, no differential expression patterns were found between both groups of patients included in the study.ConclusionAnalysis of the microRNA transcriptome profile of nonobese PLWH with severe NAFLD did not appear to differ from that of patients without NAFLD. Thus, microRNA might not serve as a proper biomarker for predicting severe NALFD in this population.

【 授权许可】

Unknown   
Copyright © 2023 Frías, Corona-Mata, Moyano, Camacho-Espejo, López-López, Caballero-Gómez, Ruiz-Cáceres, Casares-Jiménez, Pérez-Valero, Rivero-Juárez and Rivero