Evaluating the attrition, fabric integrity and insecticidal durability of two dual active ingredient nets (Interceptor® G2 and Royal® Guard): methodology for a prospective study embedded in a cluster randomized controlled trial in Benin

Malaria Journal

Methodology

Martin Akogbeto¹  Germain Gil Padonou¹  Josias Fagbohoun²  Augustin Fongnikin²  Juniace Ahoga²  Jackie Cook³  Natacha Protopopoff³  Manfred Accrombessi⁴  Corine Ngufor⁵  Abel Agbevo⁵  Thomas Syme⁵  Renaud Govoetchan⁵  Thomas S. Churcher⁶ 

[1] Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin;Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin;Panafrican Malaria Vector Research Consortium (PAMVERC), Cotonou, Benin;London School of Hygiene and Tropical Medicine (LSHTM), WC1E 7HT, London, UK;London School of Hygiene and Tropical Medicine (LSHTM), WC1E 7HT, London, UK;Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin;London School of Hygiene and Tropical Medicine (LSHTM), WC1E 7HT, London, UK;Centre de Recherches Entomologiques de Cotonou (CREC), Cotonou, Benin;Panafrican Malaria Vector Research Consortium (PAMVERC), Cotonou, Benin;MRC Centre for Global Infectious Disease Analysis, Infectious Disease Epidemiology, Imperial College London, Norfolk Place, W2 1PG, London, UK;

关键词: Durability;    Attrition;    Fabric integrity;    Bioefficacy;    Experimental hut trials;    Insecticide Treated Net;    Pyriproxyfen;    Chlorfenapyr;    Alpha-cypermethrin;    Dual active ingredient nets;    Interceptor;    Interceptor G2;    Royal Guard;   

DOI  :  10.1186/s12936-023-04708-x

 received in 2023-04-28, accepted in 2023-09-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundFollowing the World Health Organization (WHO) endorsement of dual active ingredient (AI) nets, an increased uptake of pyrethroid-chlorfenapyr and pyrethroid-pyriproxyfen nets is expected. Studies evaluating their physical and insecticidal durability are essential for making programmatic and procurement decisions. This paper describes the methodology for a prospective study to evaluate the attrition, fabric integrity, insecticidal durability of Interceptor® G2 (alpha-cypermethrin-chlorfenapyr) and Royal Guard® (alpha-cypermethrin-pyriproxyfen), compared to Interceptor® (alpha-cypermethrin), embedded in a 3-arm cluster randomized controlled trial (cRCT) in the Zou Department of Benin.MethodsTen clusters randomly selected from each arm of the cRCT will be used for the study. A total of 750 ITNs per type will be followed in 5 study clusters per arm to assess ITN attrition and fabric integrity at 6-, 12-, 24- and 36-months post distribution, using standard WHO procedures. A second cohort of 1800 nets per type will be withdrawn every 6 months from all 10 clusters per arm and assessed for chemical content and biological activity in laboratory bioassays at each time point. Alpha-cypermethrin bioefficacy in Interceptor® and Royal Guard® will be monitored in WHO cone bioassays and tunnel tests using the susceptible Anopheles gambiae Kisumu strain. The bioefficacy of the non-pyrethroid insecticides (chlorfenapyr in Interceptor® G2 and pyriproxyfen in Royal Guard®) will be monitored using the pyrethroid-resistant Anopheles coluzzii Akron strain. Chlorfenapyr activity will be assessed in tunnel tests while pyriproxyfen activity will be assessed in cone bioassays in terms of the reduction in fertility of blood-fed survivors observed by dissecting mosquito ovaries. Nets withdrawn at 12, 24 and 36 months will be tested in experimental hut trials within the cRCT study area against wild free-flying pyrethroid resistant An. gambiae sensu lato to investigate their superiority to Interceptor® and to compare them to ITNs washed 20 times for experimental hut evaluation studies. Mechanistic models will also be used to investigate whether entomological outcomes with each dual ITN type in experimental hut trials can predict their epidemiological performance in the cRCT.ConclusionThis study will provide information on the durability of two dual AI nets (Interceptor® G2 and Royal Guard®) in Benin and will help identify suitable methods for monitoring the durability of their insecticidal activity under operational conditions. The modelling component will determine the capacity of experimental hut trials to predict the epidemiological performance of dual AI nets across their lifespan.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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