Characterization of green synthesized selenium nanoparticles (SeNPs) in two different indigenous halophilic bacteria

BMC Chemistry

Research

Mohammad Reza Zolfaghari¹  Maryam Tabibi²  Soheil Aghaei²  Razieh Nazari²  Mohammad Ali Amoozegar³ 

[1] Department of Microbiology, Faculty of Basic Science, Qom Branch, Islamic Azad University, Qom, Iran;Department of Microbiology, Qom Branch, Islamic Azad University, 3749113191, Qom, Iran;Extremophiles Lab., Dept. of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran;

关键词: Biosynthesis;    Selenium nanoparticles (SeNPs);    Green synthesis;    Iran;   

DOI  :  10.1186/s13065-023-01034-w

 received in 2022-11-17, accepted in 2023-09-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundIn the biological method, using nonpathogenic and extremophile bacteria systems are not only safe and highly efficient but also a trump card for synthesizing nanoparticles. Halomonas elongata QW6 IBRC-M 10,214 (He10214) and Salinicoccus iranensis IBRC-M 10,198 (Si10198), indigenous halophilic bacteria, can be used for synthesizing selenium nanoparticles (SeNPs).MethodsSeNP biosynthesis was optimized in two halophilic bacteria and characterized by UV–Vis, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), X-ray powder diffraction (XRD), zeta potential, and energy dispersive X-ray (EDX).ResultsOptimized conditions for synthesizing SeNPs was at 300 °C at 150 rpm for 72 h and 6 mM or 8 mM concentration of Na2SeO3. UV–Vis indicated a sharp absorption peak at 294 nm. Spherical-shaped nanoparticles by a diameter of 30–100 nm were observed in FESEM and TEM microscopy images. The produced SeNPs were identified by a peak in FTIR spectra. In XRD analysis, the highest peak diffraction had a relationship with SeNPs. The zeta potential analysis showed SeNP production, and elemental selenium was confirmed by EDX.ConclusionsHalophilic bacteria, owing to easy manipulation to create optimization conditions and high resistance, could serve as appropriate organisms for the bioproduction of nanoparticles. The biological method, due to effectiveness, flexibility, biocompatibility, and low cost, could be used for the synthesis of reproducible and stable nanoparticles.

【 授权许可】

CC BY   
© Springer Nature Switzerland AG 2023

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