AMB Express | |
Optimization and characterization of antileukemic l-asparaginase produced by Fusarium solani endophyte | |
Original Article | |
Mokhtar Bishr1  Dalia A. Al-Mahdy2  Moshera M. El-Sherei2  Marwa M. Raafat3  Sarah Osama4  Osama Salama4  | |
[1] Arab Company for Pharmaceuticals and Medicinal Plants (Mepaco), Cairo, Egypt;Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, 11562, Cairo, Egypt;Microbiology and Immunology Department, Faculty of Pharmacy, Future University in Egypt, 11835, Cairo, Egypt;Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Future University in Egypt, Cairo, Egypt; | |
关键词: -asparaginase; Endophytes; Fusarium solani; Hedera helix; Optimization; Characterization; Cytotoxicity; | |
DOI : 10.1186/s13568-023-01602-2 | |
received in 2023-04-07, accepted in 2023-08-30, 发布年份 2023 | |
来源: Springer | |
【 摘 要 】
l-asparaginase is an antileukemic enzyme that hydrolyzes l-asparagine into l-aspartic acid and ammonia, causing cell starvation and apoptosis in susceptible leukemic cell populations. Currently, l-asparaginase obtained from bacterial sources is constrained by several issues, including lesser productivity, stability, selectivity, and higher toxicity. The goal of this study is to provide fungal l-asparaginase with in-vitro effectiveness towards different human carcinomas. l-asparaginase from endophytic Fusarium solani (Gene Bank accession number MW209717) isolated from the roots of the medicinal plant Hedera helix L. was characterized and optimized experimentally for maximum l-asparaginase production in addition to evaluating its subsequent cytotoxicity towards acute monocytic leukemia and human skin fibroblast cell lines. The enzyme production was maximized using potato dextrose media (15.44 IU/ml/hr) at the 5th and 6th days of fermentation with incubation temperature 30 °C, 3% asparagine, 150–180 rpm agitation rate and a 250 ml flask. Enzyme characterization studies revealed that the enzyme maintained its thermal stability with temperatures up to 60 °C. However, its optimal activity was achieved at 35 °C. On measuring the enzymatic activity at various temperatures and different pH, maximum enzyme activity was recorded at 40 °C and pH 8 using 0.1 M asparagine concentration. Results also revealed promising cytotoxic activity against acute monocytic leukemia with IC50 = 3.66 µg/ml and low cytotoxicity against tested normal human skin fibroblast cell line which suggested that it might have selective toxicity, and consequently it could be used as a less toxic alternative to the current formulations.
【 授权许可】
CC BY
© Springer-Verlag GmbH Germany, part of Springer Nature 2023
【 预 览 】
Files | Size | Format | View |
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RO202310117915141ZK.pdf | 1588KB | download | |
13690_2023_1170_Article_IEq36.gif | 1KB | Image | download |
Fig. 3 | 1390KB | Image | download |
Fig. 11 | 116KB | Image | download |
Fig. 1 | 253KB | Image | download |
Fig. 3 | 233KB | Image | download |
MediaObjects/13690_2023_1184_MOESM1_ESM.docx | 15KB | Other | download |
MediaObjects/41408_2023_912_MOESM1_ESM.pdf | 3821KB | download |
【 图 表 】
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