Disruption of the Novel Small Protein RBR7 Leads to Enhanced Plant Resistance to Blast Disease

Rice

Research

Jing Wang¹  Zhangjie Zhao¹  Xuewei Chen¹  Qing Xiong¹  Xiang Lu¹  Lian Zhou¹  Junjie Yin¹  Hui Shi¹ 

[1] State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China;

关键词: Rice blast;    Resistance;    Spontaneous lesion;    Small protein;    Salicylic acid;   

DOI  :  10.1186/s12284-023-00660-1

 received in 2023-06-29, accepted in 2023-09-14,  发布年份 2023

来源: Springer

【 摘 要 】

Plant disease is a threat to global food security. Breeding crops carrying broad-spectrum resistance loci is an effective way to control infectious disease. Disease-resistant mutants are valuable resources for deciphering the underlying mechanisms of plant immunity and could provide genetic loci to generate disease-resistant crops. Here, we identified a rice mutant, rbr7 (rice blast resistance 7), that confers resistance against different strains of Magnaporthe oryzae. Disease-mimicking necrotic lesions started to appear on the leaves of rbr7 four weeks after sowing. Histochemical analysis revealed reactive oxygen species accumulation and cell death accompanied by spontaneous lesion formation in rbr7. Map-based cloning and bulk segregation analysis showed a 2855 bp fragment deletion on chromosome 5, leading to the disruption of the LOC_Os05g28480-coding protein. Transgenic rbr7 complementation plants showed compromised resistance to rice blast, indicating that LOC_Os05g28480, or Rbr7, regulates the rice immune response. Rbr7 encodes a small protein of unknown function with 85 amino acids. Transcriptomic analysis revealed that disruption of RBR7 led to the upregulation of genes responding to salicylic acid, systemic acquired resistance and pathogenesis-related genes. Taken together, our findings reveal insights into a novel small protein involved in regulating plant resistance to rice blast and provide a potential target for crop breeding.

【 授权许可】

CC BY   
© Springer Science+Business Media, LLC, part of Springer Nature 2023

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