Plastics shape the black soldier fly larvae gut microbiome and select for biodegrading functions

Microbiome

Research

Giuseppina Sequino¹  Vincenza Cozzolino²  Edoardo Pasolli³  Francesca De Filippis³  Danilo Ercolini³  Marco Bonelli⁴  Morena Casartelli⁵  Daniele Bruno⁶  Aurora Montali⁶  Gianluca Tettamanti⁷  Marcella Reguzzoni⁸  Davide Savy⁹  Silvana Cangemi⁹  Silvia Caccia^1,10 

[1] Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy;Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy;Interdepartmental Research Centre of Nuclear Magnetic Resonance for the Environment, Agri-Food and New Materials (CERMANU), University of Naples Federico II, Portici, Italy;Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy;Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy;Department of Biosciences, University of Milan, Milan, Italy;Department of Biosciences, University of Milan, Milan, Italy;Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology (BAT Center), University of Naples Federico II, Portici, Italy;Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy;Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy;Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology (BAT Center), University of Naples Federico II, Portici, Italy;Department of Medicine and Surgery, University of Insubria, Varese, Italy;Interdepartmental Research Centre of Nuclear Magnetic Resonance for the Environment, Agri-Food and New Materials (CERMANU), University of Naples Federico II, Portici, Italy;Task Force on Microbiome Studies, University of Naples Federico II, Naples, Italy;Department of Biosciences, University of Milan, Milan, Italy;

关键词: Insect gut microbiota;    Insect gut microbiome;    Plastic biodegradation;    Hermetia illucens;    Bioconversion;   

DOI  :  10.1186/s40168-023-01649-0

 received in 2022-10-26, accepted in 2023-07-16,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundIn the last few years, considerable attention has been focused on the plastic-degrading capability of insects and their gut microbiota in order to develop novel, effective, and green strategies for plastic waste management. Although many analyses based on 16S rRNA gene sequencing are available, an in-depth analysis of the insect gut microbiome to identify genes with plastic-degrading potential is still lacking.ResultsIn the present work, we aim to fill this gap using Black Soldier Fly (BSF) as insect model. BSF larvae have proven capability to efficiently bioconvert a wide variety of organic wastes but, surprisingly, have never been considered for plastic degradation. BSF larvae were reared on two widely used plastic polymers and shotgun metagenomics was exploited to evaluate if and how plastic-containing diets affect composition and functions of the gut microbial community. The high-definition picture of the BSF gut microbiome gave access for the first time to the genomes of culturable and unculturable microorganisms in the gut of insects reared on plastics and revealed that (i) plastics significantly shaped bacterial composition at species and strain level, and (ii) functions that trigger the degradation of the polymer chains, i.e., DyP-type peroxidases, multicopper oxidases, and alkane monooxygenases, were highly enriched in the metagenomes upon exposure to plastics, consistently with the evidences obtained by scanning electron microscopy and 1H nuclear magnetic resonance analyses on plastics.ConclusionsIn addition to highlighting that the astonishing plasticity of the microbiota composition of BSF larvae is associated with functional shifts in the insect microbiome, the present work sets the stage for exploiting BSF larvae as “bioincubators” to isolate microbial strains and enzymes for the development of innovative plastic biodegradation strategies. However, most importantly, the larvae constitute a source of enzymes to be evolved and valorized by pioneering synthetic biology approaches.5errh-AhYWWkX5YWmGozvJVideo Abstract

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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