Chinese Medicine | |
Ajugol's upregulation of TFEB-mediated autophagy alleviates endoplasmic reticulum stress in chondrocytes and retards osteoarthritis progression in a mouse model | |
Research | |
Binghao Lin1  Heng Yu1  Zhao Zhang1  Zhongke Lin1  Jingtao Wu1  Jiansen Miao1  Yangcan Jin1  Jingquan Wang1  Teng Cheng1  Liwen Zhou2  | |
[1] Department of Orthopaedics, Wenzhou Key Laboratory of Perinatal Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, 325000, Wenzhou, Zhejiang Province, China;Key Laboratory of Orthopaedics of Zhejiang Province, 325000, Wenzhou, Zhejiang Province, China;The Second School of Medicine, Wenzhou Medical University, 325000, Wenzhou, Zhejiang Province, China;The First School of Medicine, Wenzhou Medical University, 325000, Wenzhou, Zhejiang Province, China; | |
关键词: Osteoarthritis; Ajugol; TBHP; TFEB; Autophagy; ER stress; DMM; | |
DOI : 10.1186/s13020-023-00824-7 | |
received in 2023-07-07, accepted in 2023-08-24, 发布年份 2023 | |
来源: Springer | |
【 摘 要 】
BackgroundOsteoarthritis (OA), a degenerative disease with a high global prevalence, is characterized by the degradation of the extracellular matrix (ECM) and the apoptosis of chondrocytes. Ajugol, a extract derived from the herb Rehmannia glutinosa, has not yet been investigated for its potential in modulating the development of OA.MethodsWe employed techniques such as western blotting, immunofluorescence, immunohistochemistry, X-ray imaging, HE staining, and SO staining to provide biological evidence supporting the role of Ajugol as a potential therapeutic agent for modulating OA. Furthermore, in an in vivo experiment, intra-peritoneal injection of 50 mg/kg Ajugol effectively mitigated the progression of OA following destabilization of the medial meniscus (DMM) surgery.ResultsOur findings revealed that treatment with 50 μM Ajugol activated TFEB-mediated autophagy, alleviating ER stress-induced chondrocyte apoptosis and ECM degradation caused by TBHP. Furthermore, in an in vivo experiment, intra-peritoneal injection of 50 mg/kg Ajugol effectively mitigated the progression of OA following destabilization of the medial meniscus (DMM) surgery.ConclusionThese results provide compelling biological evidence supporting the role of Ajugol as a potential therapeutic agent for modulating OA by activating autophagy and attenuating ER stress-induced cell death and ECM degradation. The promising in vivo results further suggest the potential of Ajugol as a treatment strategy for OA progression.
【 授权许可】
CC BY
© International Society for Chinese Medicine and BioMed Central Ltd. 2023
【 预 览 】
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MediaObjects/41408_2023_924_MOESM1_ESM.pdf | 3494KB | download | |
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