Dynamics of the cytosolic chelatable iron pool of K562 cells

FEBS Letters

Epsztejn, Silvina¹  Breuer, William¹  Ioav Cabantchik, Z.¹ 

[1] Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, 91904, Israel

关键词: Iron;    Oxidative stress;    Fluorescence;    Calcein;    Chelators;    CA;    calcein;    CA-AM;    calcein acetoxymethyl ester;    LIP;    intracellular labile iron pool;    Tf;    transferrin;    SIH;    salicylaldehyde isonicotinoyl hydrazone;    FAS;    ferrous ammonium sulfate;    FCS;    fetal calf serum;    ME;    β-mercaptoethanol;    TBHP;    tert-butylhydroperoxide;   

DOI  :  10.1016/0014-5793(96)00190-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350 nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective of 27, 65–88 and ≤17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.

【 授权许可】

Unknown