Mechanisms for leaf color changes in Osmanthus fragrans ‘Ziyan Gongzhu’ using physiology, transcriptomics and metabolomics

BMC Plant Biology

Research

Ziqi Huang¹  Yihan Wang¹  Wei Zhao¹  Nan Lin¹  Fude Shang¹  Peng Guo¹ 

[1] College of Life Science, Henan Agricultural University, 450046, Zhengzhou, Henan, China;Henan Engineering Research Center for Osmanthus Germplasm Innovation and Resource Utilization, 450046, Zhengzhou, Henan, China;

关键词: Osmanthus fragrans;    RNA-seq;    Metabolomics;    Chlorophyll;    Carotenoid;    Anthocyanin;    OfMYB;   

DOI  :  10.1186/s12870-023-04457-8

 received in 2023-03-28, accepted in 2023-09-12,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundColor-leaved O. fragrans is a variety of Osmanthus fragrans, which has both the fragrance of Osmanthus and the color of color-leaved plants. However, the molecular mechanism of color change of color-leaved O. fragrans is not clear. In this study, we analyzed the regulatory mechanism of four different color leaves of ‘Ziyan Gongzhu’ through physiological, transcriptome and metabolome levels.ResultsFirstly, we measured the leaf pigments content and leaf chromatic parameters for correlation analysis, indicating a significant correlation between them. Overall, the content of chlorophyll a + b is low and the content of anthocyanin is high in T1 and T2 leaves, along with low expression of chlorophyll synthesis genes (HEMA, CHLG, and CAO, etc.) and high expression of anthocyanin synthesis genes (F3H, F3’H, DFR and ANS, etc.), resulting purple red and light purple in T1 and T2 leaves, respectively. It was also found that the pigment closely related to the color leaves of ‘Ziyan Gongzhu’ was cyanidin. The content anthocyanins, may be regulated by two putative MYB activators (OfMYB3 and OfMYB4) and two putative MYB repressors (OfMYB1 and OfMYB2). In contrast, the content of chlorophyll a + b is high and the content of anthocyanin is low in T3 and T4 leaves, along with high expression of chlorophyll synthesis genes and low expression of anthocyanin synthesis genes, resulting yellow green and dark green in T3 and T4 leaves, respectively. And abnormal chloroplast development affects chlorophyll content in T1, T2, and T3 leaves. Although the content of carotenoids first dropped in T2 leaves, it then rapidly accumulated in T4 leaves, in sync with the increase in the expression of genes related to carotenoid biosynthesis (ZDS, LHYB, and ZEP, for example). Analysis of photosynthetic, carbohydrate and hormone-related differentially abundant metabolites (DAMs) and DEGs found that they may participate in the regulation of leaf color change of ‘Ziyan Gongzhu’ by affecting pigment synthesis.ConclusionOur results pave the way for a comprehensive knowledge of the regulatory processes governing leaf color in ‘Ziyan Gongzhu’ and identify possible genes for application regarding molecular colored-leaf cultivar breeding.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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