New investigation of anti-inflammatory activity of Polycladia crinita and biosynthesized selenium nanoparticles: isolation and characterization

Microbial Cell Factories

Research

Mostafa M. El-Sheekh¹  Mofida E. M. Makhlof²  Hend Selim³  Maisra M. El-Bouseary⁴  Alanood S. Almurshedi⁵  Basmah N. Aldosari⁵  Salma S. Almarshidy⁵  Iman M. Alfagih⁵  Bushra T. AlQuadeib⁵  Thanaa A. El-Masry⁶ 

[1] Botany Department, Faculty of Science, Tanta University, Tanta, Egypt;Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour, Egypt;Department of Biochemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt;Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Tanta University, Tanta, Egypt;Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia;Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, Egypt;

关键词: Polycladia crinita;    Macroalgae;    Brown Algae;    Selenium nanoparticles;    Anti-inflammatory;    Anti-oxidant;    Carrageenan;   

DOI  :  10.1186/s12934-023-02168-1

 received in 2023-04-24, accepted in 2023-08-07,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMarine macroalgae have gained interest recently, mostly due to their bioactive components. Polycladia crinita is an example of marine macroalgae from the Phaeophyceae class, also known as brown algae. They are characterized by a variety of bioactive compounds with valuable medical applications. The prevalence of such naturally active marine resources has made macroalgae-mediated manufacturing of nanoparticles an appealing strategy. In the present study, we aimed to evaluate the antioxidant and anti-inflammatory features of an aqueous extract of Polycladia crinita and biosynthesized P. crinita selenium nanoparticles (PCSeNPs) via a carrageenan-induced rat paw edema model. The synthesized PCSeNPs were fully characterized by UV–visible spectroscopy, FTIR, XRD, and EDX analyses.ResultsFTIR analysis of Polycladia crinita extract showed several sharp absorption peaks at 3435.2, 1423.5, and 876.4 cm−1 which represent O–H, C=O and C=C groups. Moreover, the most frequent functional groups identified in P. crinita aqueous extract that are responsible for producing SeNPs are the –NH2–, –C=O–, and –SH– groups. The EDX spectrum analysis revealed that the high percentages of Se and O, 1.09 ± 0.13 and 36.62 ± 0.60%, respectively, confirmed the formation of SeNPs. The percentages of inhibition of the edema in pretreated groups with doses of 25 and 50 mg/kg, i.p., of PCSeNPs were 62.78% and 77.24%, respectively. Furthermore, the pretreated groups with 25, 50 mg/kg of P. crinita extract displayed a substantial decrease in the MDA levels (P < 0.00, 26.9%, and 51.68% decrease, respectively), indicating potent antioxidant effect. Additionally, the pretreated groups with PCSeNPs significantly suppressed the MDA levels (P < 0.00, 54.77%, and 65.08% decreases, respectively). The results of immune-histochemical staining revealed moderate COX-2 and Il-1β expressions with scores 2 and 1 in rats pre-treated with 25 and 50 mg/kg of free extract, respectively. Additionally, the rats pre-treated with different doses of PCSeNPs demonstrated weak COX-2 and Il-1β expressions with score 1 (25 mg/kg) and negative expression with score 0 (50 mg/kg). Both antioxidant and anti-inflammatory effects were dose-dependent.ConclusionsThese distinguishing features imply that this unique alga is a promising anti-inflammatory agent. Further studies are required to investigate its main active ingredients and possible side effects.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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