Erythritol alters gene transcriptome signatures, cell growth, and biofilm formation in Staphylococcus pseudintermedius

BMC Veterinary Research

Research

Koji Nishifuji¹  Tadashi Fujii²  Takumi Tochio² 

[1] Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan;Research & Development Center, B Food Science Co., Ltd., Aichi, Japan;Department of Gastroenterology and Hepatology, Fujita Health University, Aichi, Japan;

关键词: Erythritol;    Staphylococcus pseudintermedius;    Transcriptome;    Growth inhibition;    Biofilm;   

DOI  :  10.1186/s12917-023-03711-3

 received in 2022-06-18, accepted in 2023-08-28,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundErythritol was found to inhibit the growth of microorganisms. The present study aimed to demonstrate the growth inhibition of Staphylococcus pseudintermedius by erythritol and to define the changes in gene transcription signatures induced by erythritol. Changes in the gene transcription profiles were analysed by RNA sequencing and quantitative reverse transcription PCR. Gene ontology analysis was performed to assign functional descriptions to the genes.ResultsErythritol inhibited S. pseudintermedius growth in a dose-dependent manner. We then performed a transcriptome analysis of S. pseudintermedius with and without 5% (w/w) erythritol exposure to validate the mechanism of growth inhibition. We revealed that erythritol induced up-regulation of three genes (ptsG, ppdK, and ppdkR) that are related to the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). Glucose supplementation restored the up-regulation of the PTS-related genes in response to erythritol. In addition, erythritol down-regulated eleven genes that are located in a single pur-operon and inhibited biofilm formation of S. pseudintermedius.ConclusionsThese findings indicated that erythritol antagonistically inhibits PTS-mediated glucose uptake, thereby exerting a growth inhibitory effect on S. pseudintermedius. Moreover, erythritol inhibits the ‘de novo’ IMP biosynthetic pathway that may contribute to biofilm synthesis in S. pseudintermedius.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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