High-throughput process development from gene cloning to protein production

Microbial Cell Factories

Review

Rodrigo Ledesma-Amaro¹  Alex Xiong Gao²  Manman Sun³  Zhonghu Bai⁴  Xiuxia Liu⁴  Yankun Yang⁴ 

[1] Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, SW7 2AZ, London, UK;Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, China;National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 214112, Wuxi, China;Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, SW7 2AZ, London, UK;National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 214112, Wuxi, China;Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China;Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 214122, Wuxi, China;

关键词: Recombinant protein;    Process development;    High-throughput technology;    High-throughput culture;   

DOI  :  10.1186/s12934-023-02184-1

 received in 2023-05-24, accepted in 2023-08-19,  发布年份 2023

来源: Springer

【 摘 要 】

In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for a given application is also increasing. Faced with peer competition, budgetary, and time constraints, companies and laboratories must find ways to develop a robust manufacturing process for recombinant protein production. In this review, we explore high-throughput technologies for recombinant protein expression and present a holistic high-throughput process development strategy that spans from genes to proteins. We discuss the challenges that come with this task, the limitations of previous studies, and future research directions.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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