RNA-Seq analysis of the pyloric caecum, liver, and muscle reveals molecular mechanisms regulating fillet color in rainbow trout

BMC Genomics

Research

Tim Leeds¹  Ali Ali²  Mohamed Salem²  Ridwan O. Ahmed² 

[1] Department of Agriculture Kearneysville, National Center for Cool and Cold Water Aquaculture, United States, Agricultural Research Service, 25430, Kearneysville, WV, USA;Department of Animal and Avian Sciences, University of Maryland, 20742, College Park, MD, USA;

关键词: Aquaculture;    Antioxidant;    Beta-carotene;    Micellization;    Pathways;    Pigmentation;   

DOI  :  10.1186/s12864-023-09688-5

 received in 2023-05-18, accepted in 2023-09-20,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe characteristic pink-reddish color in the salmonids fillet is an important, appealing quality trait for consumers and producers. The color results from diet supplementation with carotenoids, which accounts for up to 20–30% of the feed cost. Pigment retention in the muscle is a highly variable phenotype. In this study, we aimed to understand the molecular basis for the variation in fillet color when rainbow trout (Oncorhynchus mykiss) fish families were fed an Astaxanthin-supplemented diet. We used RNA-Seq to study the transcriptome profile in the pyloric caecum, liver, and muscle from fish families with pink-reddish fillet coloration (red) versus those with lighter pale coloration (white).ResultsMore DEGs were identified in the muscle (5,148) and liver (3,180) than in the pyloric caecum (272). Genes involved in lipid/carotenoid metabolism and transport, ribosomal activities, mitochondrial functions, and stress homeostasis were uniquely enriched in the muscle and liver. For instance, the two beta carotene genes (BCO1 and BCO2) were significantly under-represented in the muscle of the red fillet group favoring more carotenoid retention. Enriched genes in the pyloric caecum were involved in intestinal absorption and transport of carotenoids and lipids. In addition, the analysis revealed the modulation of several genes with immune functions in the pyloric caecum, liver, and muscle.ConclusionThe results from this study deepen our understanding of carotenoid dynamics in rainbow trout and can guide us on strategies to improve Astaxanthin retention in the rainbow trout fillet.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 1

Fig. 5

13690_2023_1170_Article_IEq77.gif

12888_2023_5142_Article_IEq14.gif

13690_2023_1170_Article_IEq74.gif

12951_2023_2095_Article_IEq12.gif

12951_2023_2095_Article_IEq11.gif

13690_2023_1170_Article_IEq72.gif

Fig. 3

13690_2023_1170_Article_IEq172.gif

Fig. 8

40798_2023_628_Figd_HTML.png