LuxR402 of Novosphingobium sp. HR1a regulates the correct configuration of cell envelopes

Frontiers in Microbiology

Microbiology

Ana Segura¹  Lázaro Molina² 

[1] Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Madrid, Spain;null;

关键词: LuxR;    flocculation;    membrane envelop;    nutrient starvation;    stress;   

DOI  :  10.3389/fmicb.2023.1205860

 received in 2023-04-14, accepted in 2023-07-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Although there is some evidence to suggest that LuxR-solo proteins participate in inter-species or even inter-kingdom communication, most of the LuxR-solo protein functions are unknown. We have characterized the LuxR402 regulator of Novosphingobium sp. HR1a, a bacterial strain with the ability to establish high numbers in the plant rhizosphere and able to degrade a wide range of polycyclic aromatic hydrocarbons. LuxR402 controls the aggregation state of the bacterial culture; cultures of a mutant strain lacking this regulator flocculate in less than 3 h without agitation. We have demonstrated that the bacterial surface of the mutant is highly hydrophobic and that the mutant cells assimilate sugars slower than the wild-type. The flocculation mechanism has been demonstrated to be involved in the survival of the strain under unfavorable conditions; the luxR402 gene is repressed and produces flocculation in the presence of salicylate, a substrate that, although being assimilated by Novosphingobium, is toxic to cells at high concentrations. The flocculation of cultures in industrial setups has mainly been achieved through the addition of chemicals; these studies open up the possibility of controlling the flocculation by regulating the level of expression of the luxR402 gene.

【 授权许可】

Unknown   
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