Small Trafficking Inhibitor Retro-2 Disrupts the Microtubule-Dependent Trafficking of Autophagic Vacuoles

Frontiers in Cell and Developmental Biology

Vanessa Lievin-Le Moal¹  Valérie Nicolas² 

[1] University Paris-Saclay, Inserm, UMR-S 996 Inflammation, Microbiome and Immunosurveillance, Clamart, France;Université Paris-Saclay, Institut Paris-Saclay d’Innovation Thérapeutique (IPSIT), Microscope Facility (MIPSIT), UMS–US31–UMS3679, Châtenay-Malabry, France;

关键词: autophagy;    nutrient starvation;    microtubules;    vacuolar trafficking;    autophagosome;    autolysosome;   

DOI  :  10.3389/fcell.2020.00464

来源: DOAJ

【 摘 要 】

Autophagy is a catabolic recycling process by which a cell degrades its own constituents to contribute to cell homeostasis or survival. We report that the small trafficking inhibitor Retro-2 impairs microtubule-dependent vacuolar trafficking in autophagy. Retro-2 induced autophagy and promoted the dramatic cytoplasmic accumulation of large autophagosomes. Moreover, Retro-2 decreased the spreading of autophagosomes within the cytoplasm of nutrient-starved cells. In addition, Retro-2 abolished autolysosomes formation. We show that these effects arise from hitherto unsuspected disassembly activity of the small molecule on the cellular microtubule network, which is known to act as a key regulator of vacuolar trafficking of the autophagy pathway.

【 授权许可】

Unknown