The phosphatidylinositol 3-phosphate effector FYVE3 regulates FYVE2-dependent autophagy in Arabidopsis thaliana

Frontiers in Plant Science

Plant Science

Jeong Hun Kim¹  Han Nim Lee¹  Taijoon Chung¹  Hyera Jung¹  Kyoungjun Song¹ 

[1] Department of Biological Sciences, Pusan National University, Busan, Republic of Korea;

关键词: ATG8;    autophagosome;    autophagy;    phosphatidylinositol 3-phosphate;    phosphoinositide;    vacuolar trafficking;   

DOI  :  10.3389/fpls.2023.1160162

 received in 2023-02-06, accepted in 2023-02-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Phosphatidylinositol 3-phosphate (PI3P) is a signaling phospholipid that play a key role in endomembrane trafficking, specifically autophagy and endosomal trafficking. However, the mechanisms underlying the contribution of PI3P downstream effectors to plant autophagy remain unknown. Known PI3P effectors for autophagy in Arabidopsis thaliana include ATG18A (Autophagy-related 18A) and FYVE2 (Fab1p, YOTB, Vac1p, and EEA1 2), which are implicated in autophagosome biogenesis. Here, we report that FYVE3, a paralog of plant-specific FYVE2, plays a role in FYVE2-dependent autophagy. Using yeast two-hybrid and bimolecular fluorescence complementation assays, we determined that the FYVE3 protein was associated with autophagic machinery containing ATG18A and FYVE2, by interacting with ATG8 isoforms. The FYVE3 protein was transported to the vacuole, and the vacuolar delivery of FYVE3 relies on PI3P biosynthesis and the canonical autophagic machinery. Whereas the fyve3 mutation alone barely affects autophagic flux, it suppresses defective autophagy in fyve2 mutants. Based on the molecular genetics and cell biological data, we propose that FYVE3 specifically regulates FYVE2-dependent autophagy.

【 授权许可】

Unknown   
Copyright © 2023 Kim, Jung, Song, Lee and Chung