Metabolic impact of polyphenol-rich aronia fruit juice mediated by inflammation status of gut microbiome donors in humanized mouse model

Frontiers in Nutrition

Nutrition

Carl J. Yeoman¹  Brian Bothner²  Jesse T. Peach²  Hunter Fausset²  Stephanie M. G. Wilson³  Mary P. Miles³  Seth T. Walk⁴  Zachary T. Miller⁵ 

[1] Department of Animal and Range Sciences, Montana State University, Bozeman, MT, United States;Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, United States;Department of Food Systems, Nutrition, and Kinesiology, Montana State University, Bozeman, MT, United States;Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, United States;Department of Research Centers, Montana State University, Bozeman, MT, United States;

关键词: gastrointestinal microbiome;    high fat diet;    inflammation;    functional food;    metabolomics;   

DOI  :  10.3389/fnut.2023.1244692

 received in 2023-06-22, accepted in 2023-08-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundThe Aronia melanocarpa fruit is emerging as a health food owing to its high polyphenolic content and associated antioxidant activity. Antioxidant-rich foods, such as Aronia fruit, may counter inflammatory stimuli and positively modulate the gut microbiome. However, a comprehensive study characterizing the impact of Aronia fruit supplementation has not been completed. Therefore, we completed analyses measuring the metabolic, microbial, and inflammatory effects of a diet supplemented with Aronia fruit juice.MethodHumanized mice were generated by colonizing gnotobiotic mice with microbiomes from human donors presenting disparate inflammation levels. Blood and fecal samples were collected throughout the course of an 8-week dietary intervention with either Aronia juice or a carbohydrate-matched beverage alone (2 weeks) or in combination with a high-fat diet to induce inflammation (6 weeks). Samples were analyzed using 16S rRNA gene sequencing (stool) and liquid chromatography-mass spectrometry (serum).ResultsWe demonstrated transfer of microbiome composition and diversity and metabolic characteristics from humans with low and high inflammation levels to second-generation humanized mice. Aronia supplementation provided robust protection against high-fat diet induced metabolic and microbiome changes that were dependent in part on microbiome donor. Aronia induced increases in bacteria of the Eggerthellaceae genus (7-fold) which aligns with its known ability to metabolize (poly)phenols and in phosphatidylcholine metabolites which are consistent with improved gut barrier function. The gut microbiome from a low inflammation phenotype donor provided protection against high-fat diet induced loss of microbiome β-diversity and global metabolomic shifts compared to that from the high inflammation donor.ConclusionThese metabolic changes elucidate pathway-specific drivers of reduced inflammation stemming from both Aronia and the gut microbiota.

【 授权许可】

Unknown   
Copyright © 2023 Wilson, Peach, Fausset, Miller, Walk, Yeoman, Bothner and Miles.