Anti-Inflammatory Activity of Haskap Cultivars is Polyphenols-Dependent

Biomolecules

H. P. Vasantha Rupasinghe²  Mannfred M. A. Boehm²  Satvir Sekhon-Loodu²  Indu Parmar²  Bob Bors⁴  Andrew R. Jamieson³  Michael Breitenbach¹ 

[1] Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada;;Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada; E-Mails:;Agriculture and Agri-Food Canada, Kentville, NS B4N 1J5, Canada; E-Mail:;Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada; E-Mail:

关键词: haskap;    inflammation;    cytokines;    polyphenols;    macrophages;    functional food;   

DOI  :  10.3390/biom5021079

来源: mdpi

【 摘 要 】

Haskap (Lonicera caerulea L.) berries have long been used for their health promoting properties against chronic conditions. The current study investigated the effect of Canadian haskap berry extracts on pro-inflammatory cytokines using a human monocytic cell line THP-1 derived macrophages stimulated by lipopolysaccharide. Methanol extracts of haskap from different growing locations in Canada were prepared and characterized for their total phenolic profile using colorimetric assays and liquid chromatography—Mass spectrometry (UPLC-MS/MS). Human THP-1 monocytes were seeded in 24-well plates (5 × 10⁵/well) and treated with phorbol 12-myristate 13-acetate (PMA, 0.1 μg/mL) for 48 h to induce macrophage differentiation. After 48 h, the differentiated macrophages were washed with Hank’s buffer and treated with various concentrations of test compounds for 4 h, followed by the lipopolysaccharide (LPS)-stimulation (18 h). Borealis cultivar showed the highest phenolic content, flavonoid content and anthocyanin content (p < 0.05). A negative correlation existed between the polyphenol concentration of the extracts and pro-inflammatory cytokines: Interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-α), prostaglandin (PGE₂), and cyclooxygenase-2 (COX-2) enzyme. Borealis exhibited comparable anti-inflammatory effects to COX inhibitory drug, diclofenac. The results showed that haskap berry polyphenols has the potential to act as an effective inflammation inhibitor.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.