Frequent IgE recognition of Blomia tropicalis allergen molecules in asthmatic children and young adults in equatorial Africa

Frontiers in Immunology

Immunology

Susanne Vrtala¹  Nishelle D’souza¹  Christian Lupinek¹  Huey-Jy Huang¹  Rudolf Valenta²  Gabrielle Pauli³  Antoine Roos³  Carole Wurmser³  Cosme Kokou⁴  Nathalie Acevedo⁵  Ronald Regino⁵  Luis Caraballo⁵  Josefina Zakzuk⁵ 

[1] Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria;Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria;National Research Center, Institute of Immunology FMBA of Russia, Moscow, Russia;Laboratory for Immunopathology, Department of Clinical Immunology and Allergy, Sechenov First Moscow State Medical University, Moscow, Russia;Karl Landsteiner University of Health Sciences, Krems, Austria;Faculty of Medicine, University Louis Pasteur, Strasbourg, France;Hôpital Albert Schweitzer, Lambaréné, Gabon;Hôpital Albert Schweitzer, Lambaréné, Gabon;Institute for Immunological Research, Universidad de Cartagena, Cartagena, Colombia;

关键词: allergy;    allergens;    asthma;    equatorial Africa;    allergen microarray;    Blomia tropicalis;    Dermatophagoides;   

DOI  :  10.3389/fimmu.2023.1133935

 received in 2022-12-29, accepted in 2023-05-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundAsthma is not well investigated in equatorial Africa and little is known about the disease-associated allergen molecules recognized by IgE from patients in this area. The aim was to study the molecular IgE sensitization profile of asthmatic children and young adults in a semi-rural area (Lambaréné) of an equatorial African country (Gabon), to identify the most important allergen molecules associated with allergic asthma in equatorial Africa.MethodsFifty-nine asthmatic patients, mainly children and few young adults, were studied by skin prick testing to Dermatophagoides pteronyssinus (Der p), D. farinae (Der f), cat, dog, cockroach, grass, Alternaria and peanut. Sera were obtained from a subset of 35 patients, 32 with positive and 3 with negative skin reaction to Der p and tested for IgE reactivity to 176 allergen molecules from different allergen sources by ImmunoCAP ISAC microarray technology and to seven recombinant Blomia tropicalis (Blo t) allergens by IgE dot blot assay.ResultsThirty-three of the 59 patients (56%) were sensitized to Der p and 23 of them (39%) were also sensitized to other allergen sources, whereas 9 patients (15%) were only sensitized to allergen sources other than Der p. IgE serology analyses (n=35) showed high IgE-binding frequencies to the Blo t allergens Blo t 5 (43%), Blo t 21 (43%) and Blo t 2 (40%), whereas the Der p allergens rDer p 2, rDer p 21 and rDer p 5 (34%, 29% and 26%) were less frequently recognized. Only few patients showed IgE reactivity to allergens from other allergen sources, except to allergens containing carbohydrate determinants (CCDs) or to wasp venom allergens (i.e., antigen 5).ConclusionOur results thus demonstrate that IgE sensitization to mite allergens is very prevalent in asthmatics in Equatorial Africa with B. tropicalis allergen molecules representing the most important ones associated with allergic asthma.

【 授权许可】

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Copyright © 2023 Pauli, Wurmser, Roos, Kokou, Huang, D’souza, Lupinek, Zakzuk, Regino, Acevedo, Caraballo, Vrtala and Valenta