Gut microbiota and atopic dermatitis: a two-sample Mendelian randomization study

Frontiers in Medicine

Medicine

Yan Xue¹  Han Wang¹  Jiang Xie¹  Yajun Chen²  Linzhu Zhang³ 

[1] The Third People’s Hospital of Chengdu, Clinical College of Southwest Jiao Tong University, Chengdu, China;The Third People’s Hospital of Chengdu, Clinical College of Southwest Jiao Tong University, Chengdu, China;North Sichuan Medical College, Nanchong, Sichuan, China;The Third People’s Hospital of Chengdu, Clinical College of Southwest Jiao Tong University, Chengdu, China;School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, China;

关键词: gut flora;    gut microbiota;    atopic dermatitis;    Mendelian randomization;    single nucleotide polymorphisms;   

DOI  :  10.3389/fmed.2023.1174331

 received in 2023-02-26, accepted in 2023-06-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundAccumulating evidence suggests that alterations in gut microbiota composition and diversity are associated with Atopic dermatitis (AD). But until now, the causal association between them has been unclear.MethodsWe employed a two-sample Mendelian Randomization (MR) study to estimate the potential causality of gut microbiota on AD risk. The summary statistics related to the gut microbiota were obtained from a large-scale genome-wide genotype and 16S fecal microbiome dataset from 18,340 individuals (24 cohorts) analyzed by the MiBioGen Consortium, comprising 211 gut microbiota. AD data were also derived from strictly defined AD data collected by FinnGen biobank analysis, which included 218,467 European ancestors (5,321 AD patients and 213,146 controls). The inverse variance weighted method (IVW), weighted median (WME), and MR-Egger were used to determine the changes of AD pathogenic bacterial taxa, followed by sensitivity analysis including horizontal pleiotropy analysis, Cochran’s Q test, and the leave-one-out method to assess the reliability of the results. In addition, MR Steiger’s test was used to test the suppositional relationship between exposure and outcome.ResultsA total of 2,289 SNPs (p < 1 × 10−5) were included, including 5 taxa and 17 bacterial characteristics (1 phylum, 3 classes, 1 order, 4 families, and 8 genera), after excluding the IVs with linkage disequilibrium (LD). Combining the analysis of the results of the IVW models, there were 6 biological taxa (2 families, and 4 genera) of the intestinal flora positively associated with the risk of AD and 7 biological taxa (1 phylum, 2 classes, 1 order, 1 family, and 2 genera) of the intestinal flora negatively associated. The IVW analysis results showed that Tenericutes, Mollicutes, Clostridia, Bifidobacteriaceae, Bifidobacteriales, Bifidobacterium, and Christensenellaceae R 7 group were negatively correlated with the risk of AD, while Clostridiaceae 1, Bacteroidaceae, Bacteroides, Anaerotruncus, the unknown genus, and Lachnospiraceae UCG001 showed the opposite trend. And the results of the sensitivity analysis were robust. MR Steiger’s test showed a potential causal relationship between the above intestinal flora and AD, but not vice versa.ConclusionThe present MR analysis genetically suggests a causal relationship between changes in the abundance of the gut microbiota and AD risk, thus not only providing support for gut microecological therapy of AD but also laying the groundwork for further exploration of the mechanisms by which the gut microbiota contributes to the pathogenesis of AD.

【 授权许可】

Unknown   
Copyright © 2023 Xue, Zhang, Chen, Wang and Xie.