Development of a new cellular immunological detection method for tuberculosis based on HupB protein induced IL-6 release test

Frontiers in Microbiology

Microbiology

Shimin Wu¹  Xuefang Li²  Haoming Wu²  Huaiheng Zhou²  Ye Zhang²  Xiaoli Yu²  Shengsheng Jiang²  Peng Hu²  Yutong Wei²  Hualin Wang²  Yang Li²  Shulin Zhang³ 

[1] Center for Clinical Laboratory, General Hospital of the Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, China;School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China;School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China;Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China;

关键词: tuberculosis;    HupB;    IL-6;    PBMCs;    IGRA;   

DOI  :  10.3389/fmicb.2023.1148503

 received in 2023-01-20, accepted in 2023-03-14,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectiveAs a virulence factor, HupB plays important roles in the survival of MTB after infection and modulates the host immune response. In the current study, we aim to explore a new cellular immunological detection method for tuberculosis infection detection based on HupB protein.MethodsHupB was used to stimulate PBMCs extracted from pulmonary tuberculosis (PTB) patients, and secreted cytokines was examined. Then, we constructed a single center and a multi-center clinical trials to collect PBMCs from PTB patients, nPTB patients, or healthy volunteers to verify our findings.ResultsCytokine’s screening illustrated that IL-6 was the only cytokine released after HupB stimulation. Single-center and multi-center clinical trials showed that HupB stimulation significantly increased the level of IL-6 in the supernatant of PBMCs from PTB patients. Then we compared the specificity and sensitivity of HupB induced IL-6 release assay with ESAT-6 and CFP10 induced interferon γ release assay (IGRA), and found in smear positive PTB patients, the specificity and sensitivity of HupB induced IL-6 release assay was better than IGRA, and in smear negative PTB patients, the sensitivity was better. Combination of both assays provided an improved specificity and sensitivity for tuberculosis diagnosis.ConclusionThis study explored an immunological detection method for tuberculosis infection cells based on HupB protein-induced IL-6 release test, which can be used to enhance the diagnosis diagnostic accuracy of TB.

【 授权许可】

Unknown   
Copyright © 2023 Yu, Jiang, Li, Zhou, Wei, Li, Zhang, Hu, Wu, Wang, Wu and Zhang.