Microbial community succession in the intestine of mice with deep partial-thickness burns

Frontiers in Microbiology

Microbiology

Xiao-Li Xie¹  Jian-Zheng Yang²  Jia-Hao Zeng²  Jing-Tao Xu²  Qi Wang²  Kai-Kai Zhang²  Xiu-Wen Li²  Li-Jian Chen²  Yi Liu²  Yan Lin²  Jing-Wen Yang²  Long Chen²  Jia-Hao Li²  Jia-Li Liu²  Clare Hsu² 

[1] Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Toxicology, School of Public Health, Southern Medical University, Guangzhou, Guangdong, China;Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, China;

关键词: deep partial-thickness burn;    16S rRNA sequencing;    microbial dysbiosis;    microbial community succession;    microbial diversity;   

DOI  :  10.3389/fmicb.2023.1140440

 received in 2023-01-11, accepted in 2023-04-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionBurn injury has been shown to lead to changes in the composition of the gut microbiome and cause other damage in patients. However, little is known about how the gut microbial community evolves in individuals who have recovered from burn injury.MethodsIn this study, we established a model of deep partial-thickness burn in mice and collected fecal samples at eight time points (pre-burn, 1, 3, 5, 7, 14, 21, and 28 days post-burn) for 16S rRNA amplification and high-throughput sequencing.ResultsThe results of the sequencing were analyzed using measures of alpha diversity, and beta diversity and taxonomy. We observed that the richness of the gut microbiome declined from day 7 post-burn and that the principal component and microbial community structure varied over time. On day 28 after the burn, the microbiome composition largely returned to the pre-burn level, although day 5 was a turning point for change. Some probiotics, such as the Lachnospiraceae_NK4A136_group, decreased in composition after the burn but were restored in the later recovery period. In contrast, Proteobacteria showed an opposite trend, which is known to include potential pathogenic bacteria.ConclusionThese findings demonstrate gut microbial dysbiosis after burn injury and provide new insights into the burn-related dysbiosis of the gut microbiome and strategies for improving the treatment of burn injury from the perspective of the microbiota.

【 授权许可】

Unknown   
Copyright © 2023 Chen, Liu, Yang, Lin, Hsu, Zhang, Liu, Li, Li, Yang, Chen, Zeng, Xie, Xu and Wang.