Advancements in ToF-SIMS imaging for life sciences

Frontiers in Chemistry

Chemistry

Yao Zhao¹  Feifei Jia²  Xia Zhao² 

[1]Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China

[2]National Institutes for Food and Drug Control, Beijing, China

关键词: ToF-SIMS;    life science;    metabolomics;    lipidomics;    single cell imaging;   

DOI  :  10.3389/fchem.2023.1237408

 received in 2023-06-09, accepted in 2023-08-14,  发布年份 2023

来源: Frontiers

【 摘 要 】

In the last 2 decades, Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) has gained significant prominence as a powerful imaging technique in the field of life sciences. This comprehensive review provides an in-depth overview of recent advancements in ToF-SIMS instrument technology and its applications in metabolomics, lipidomics, and single-cell analysis. We highlight the use of ToF-SIMS imaging for studying lipid distribution, composition, and interactions in cells and tissues, and discuss its application in metabolomics, including the analysis of metabolic pathways. Furthermore, we review recent progress in single-cell analysis using ToF-SIMS, focusing on sample preparation techniques, in situ investigation for subcellular distribution of drugs, and interactions between drug molecules and biological targets. The high spatial resolution and potential for multimodal analysis of ToF-SIMS make it a promising tool for unraveling the complex molecular landscape of biological systems. We also discuss future prospects and potential advancements of ToF-SIMS in the research of life sciences, with the expectation of a significant impact in the field.

【 授权许可】

Unknown   
Copyright © 2023 Jia, Zhao and Zhao.