Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos

Frontiers in Veterinary Science

Veterinary Science

Montaser Elsayed Ali¹  Magdy R. Badr²  Ahmed Ezzat Ahmed³  Khalid M. Al Syaad⁴  Fatimah A. Al-Saeed⁵  Ramya Ahmad Sindi⁶  Hassan A. Hussein⁷  Ahmed Saad A. Hassaneen⁸  Nasra Ahmed Yousef⁸  Mohamed Abdelrahman⁹ 

[1] Animal Production Department, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt;Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Al Haram, Giza, Egypt;Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia;Department of Theriogenology, Obstetrics, and Artificial Insemination, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt;Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia;Director of the Research Center, Faculty of Science, King Khalid University, Abha, Saudi Arabia;Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia;Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha, Saudi Arabia;Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Mecca, Saudi Arabia;Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt;Department of Theriogenology, Obstetrics, and Artificial Insemination, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt;Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China;Animal Production Department, Faculty of Agriculture, Assuit University, Assiut, Egypt;

关键词: buffalo;    IVF;    EGF;    βME;    fertilization;    embryo production;    oocyte;   

DOI  :  10.3389/fvets.2023.1138220

 received in 2023-01-05, accepted in 2023-02-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the in vitro maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities.

【 授权许可】

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Copyright © 2023 Ahmed, Sindi, Yousef, Hussein, Badr, Syaad, Al-Saeed, Hassaneen, Abdelrahman and Ali.