Rapid diagnosis of fatal Nocardia kroppenstedtii bacteremic pneumonia and empyema thoracis by next-generation sequencing: a case report

Frontiers in Medicine

Medicine

Fanfan Xing¹  Simon K. F. Lo¹  Qianyun Lu¹  Yao Xia²  Susanna K. P. Lau²  Patrick C. Y. Woo³ 

[1] Department of Clinical Microbiology and Infection Control, The University of Hong Kong—Shenzhen Hospital, Shenzhen, China;Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China;Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China;Doctoral Program in Translational Medicine and Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan;The iEGG and Animal Biotechnology Research Center, National Chung Hsing University, Taichung, Taiwan;

关键词: Nocardia kroppenstedtii;    immunocompromised patients;    next-generation sequencing;    Oxford Nanopore;    MinION;   

DOI  :  10.3389/fmed.2023.1226126

 received in 2023-05-20, accepted in 2023-06-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

Nocardia species do not replicate as rapidly as other pyogenic bacteria and nocardial infections can be highly fatal, particularly in immunocompromised patients. Here, we present the first report of fatal Nocardia kroppenstedtii bacteremic pneumonia and empyema thoracis diagnosed by next-generation sequencing (NGS) using the Oxford Nanopore Technologies' MinION device. The bacterium was not identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Due to its low equipment cost, short turn-around-time, and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.

【 授权许可】

Unknown   
Copyright © 2023 Xing, Xia, Lu, Lo, Lau and Woo.