The first rare case of Candida palmioleophila infection reported in China and its genomic evolution in a human host environment

Frontiers in Microbiology

Microbiology

Na Wu¹  Jingping Zhang¹  Zhihui Ren²  Chen Rong³  Min Yang³  Yunzhuo Chu³  Yusheng Wu³  Hailong Li³  Sufei Tian³  Yanyan Jiang³  Ning Jiang³  Jingjing Chen³ 

[1] Department of Infectious Diseases, The First Hospital of China Medical University, Shenyang, China;Department of Intensive Care Unit, Shenyang Fourth People’s Hospital of China Medical University, Shenyang, China;National Clinical Research Center for Laboratory Medicine, Department of Laboratory Medicine, The First Hospital of China Medical University, Shenyang, China;

关键词: Candida palmioleophila;    yeast;    whole-genome sequence;    ERG11;    ITS;   

DOI  :  10.3389/fmicb.2023.1165721

 received in 2023-02-14, accepted in 2023-07-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionCandida palmioleophila is a rare human pathogenic fungus, which has been poorly characterized at the genome level. In this study, we reported the first fatal case of C. palmioleophila infection in China and investigate the microevolution of C. palmioleophila in the human host environment.MethodsA series of C. palmioleophila stains were collected from the patient at different time points for routine microbial and drug sensitivity testing. The first C. palmioleophila isolate 07202534 was identified by de novo whole genome sequencing. The in vitro and in vivo genetic evolutionary characteristics of C. palmioleophila were discussed based on the analysis of bioinformatics data.ResultsThe six C. palmioleophila isolates displayed dose-dependent sensitivity to fluconazole. The C. palmioleophila genome contained homologous genes such as CDR1 and MDR1, which were recognized to be related to azole resistance. In addition, amino acid variation was detected at F105L and other important sites of ERG11. In addition, the mean divergence time between C. palmioleophila and Scheffersomyces stipites CBS 6054 was 406.04 million years, indicating that C. palmioleophila originated earlier than its closest relative. In addition, the six strains of C. palmioleophila isolated form the patient had higher homology and fewer mutation sites, which indicated the stability in C. palmioleophila genome. We also found that C. palmioleophila had a wide natural niche and may evolve slowly.DiscussionWe believe that this study will contribute to improve our understanding of the genetic evolution, pathogenicity, and drug resistance of C. palmioleophila and will aid in the prevention and control of its spread.

【 授权许可】

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Copyright © 2023 Wu, Wu, Chu, Ren, Li, Rong, Yang, Jiang, Jiang, Chen, Zhang and Tian.