Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila

Genetics and Molecular Biology

Adolfo Jose Mota²  Graziella Nuernberg Back-brito¹  Francisco G. Nobrega¹ 

[1] ,Universidade de São Paulo Instituto de Biociências

关键词: Candida palmioleophila;    Debaryomyces hansenii;    differential identification;    Pichia guilliermondii;    real-time PCR;   

DOI  :  10.1590/S1415-47572011005000059

来源: SciELO

【 摘 要 】

Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.

【 授权许可】

CC BY   
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