Intra-osseous plasma rich in growth factors enhances cartilage and subchondral bone regeneration in rabbits with acute full thickness chondral defects: Histological assessment

Frontiers in Veterinary Science

Veterinary Science

Deborah Chicharro¹  Elena Damia¹  Pau Pelaez¹  Laura Miguel-Pastor¹  Ayla del Romero¹  José M. Carrillo¹  Mónica Rubio¹  Joaquín J. Sopena¹  Marta Torres-Torrillas¹ 

[1] Bioregenerative Medicine and Applied Surgery Research Group, Department of Animal Medicine and Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, Spain;García Cugat Foundation CEU-UCH Chair of Medicine and Regenerative Surgery, CEU Cardenal Herrera University, CEU Universities, Valencia, Spain;

关键词: platelet rich plasma;    growth factors;    osteoarthritis;    chondral defect;    articular cartilage;    histology;    bioregenerative therapies;   

DOI  :  10.3389/fvets.2023.1131666

 received in 2022-12-25, accepted in 2023-03-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundIntra-articular (IA) combined with intra-osseous (IO) infiltration of plasma rich in growth factors (PRGF) have been proposed as an alternative approach to treat patients with severe osteoarthritis (OA) and subchondral bone damage. The aim of the study is to evaluate the efficacy of IO injections of PRGF to treat acute full depth chondral lesion in a rabbit model by using two histological validated scales (OARSI and ICRS II).MethodologyA total of 40 rabbits were included in the study. A full depth chondral defect was created in the medial femoral condyle and then animals were divided into 2 groups depending on the IO treatment injected on surgery day: control group (IA injection of PRGF and IO injection of saline) and treatment group (IA combined with IO injection of PRGF). Animals were euthanized 56 and 84 days after surgery and the condyles were processed for posterior histological evaluation.ResultsBetter scores were obtained in treatment group in both scoring systems at 56- and 84-days follow-up than in control group. Additionally, longer-term histological benefits have been obtained in the treatment group.ConclusionsThe results suggests that IO infiltration of PRGF enhances cartilage and subchondral bone healing more than the IA-only PRGF infiltration and provides longer-lasting beneficial effects.

【 授权许可】

Unknown   
Copyright © 2023 Torres-Torrillas, Damia, Romero, Pelaez, Miguel-Pastor, Chicharro, Carrillo, Rubio and Sopena.