Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Mehmet Can Ulucesme¹  Munir Aktas¹  Sezayi Ozubek¹  Ferda Sevinc²  Onur Ceylan²  Shinuo Cao³  Mo Zhou⁴  Xuenan Xuan⁵ 

[1] Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, Türkiye;Department of Parasitology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Türkiye;Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China;Jiangsu Key Laboratory for High-tech Research and Development of Veterinary Biopharmaceuticals, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, China;National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan;

关键词: BoSA1;    cross-reaction;    diagnosis;    parasitemia;    sandwich ELISA;   

DOI  :  10.3389/fcimb.2023.1238369

 received in 2023-06-11, accepted in 2023-07-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Ovine babesiosis caused by Babesia ovis is an economically significant disease. Recently, a few B. ovis-specific proteins, including recombinant B. ovis secreted antigen-1 (rBoSA1), have been identified. Immunological analyses revealed that rBoSA1 resides within the cytoplasm of infected erythrocytes and exhibits robust antigenic properties for detecting anti-B. ovis antibodies. This protein is released into the bloodstream during the parasite’s development. It would be possible to diagnose active infections by detecting this secretory protein. For this purpose, a rBoSA1-specific polyclonal antibody-based sandwich ELISA was optimized in this study. Blood samples taken from the naturally (n: 100) and experimentally (n: 15) infected sheep were analyzed for the presence of native BoSA1. The results showed that native BoSA1 was detectable in 98% of naturally infected animals. There was a positive correlation between parasitemia level in microscopy and protein density in sandwich ELISA. Experimentally infected animals showed positive reactions from the first or second day of inoculations. However, experimental infections carried out by Rhipicephalus bursa ticks revealed the native BoSA1 was detectable from the 7th day of tick attachment when the parasite began to be seen microscopically. Sandwich ELISA was sensitive enough to detect rBoSA1 protein at a 1.52 ng/ml concentration. Additionally, no serological cross-reactivity was observed between animals infected with various piroplasm species, including Babesia bovis, B. bigemina, B. caballi, B. canis, B. gibsoni, Theileria equi, and T. annulata. Taken collectively, the findings show that the rBoSA1-specific polyclonal antibody-based sandwich ELISA can be successfully used to diagnose clinical B. ovis infections in sheep at the early stage.

【 授权许可】

Unknown   
Copyright © 2023 Sevinc, Zhou, Cao, Ceylan, Ulucesme, Ozubek, Aktas and Xuan

【 图 表 】

fcimb-13-1238369-i001.tif