Inhibitory effects of Patchouli alcohol on the early lifecycle stages of influenza A virus

Frontiers in Microbiology

Microbiology

Jielan Lai¹  Zhongde Zhang²  Xiaohong Liu³  Huiting Huang³  Shaofeng Zhan³  Ziren Su⁴  Pan Pan⁵  Qiong Zhang⁶  Haishan Long⁷  Geng Li⁷  Wen Zhang⁷  Yaohua Fan⁸  Yanni Lai⁹ 

[1] Cancer Prevention and Treatment Center, Sun Yat-sen University, Guangzhou, China;Department of Emergency, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China;Department of Pneumology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China;Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Guangzhou University of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou, China;Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, The First Affiliated Hospital of Jinan University, Guangzhou, China;Guangzhou Laboratory, Guangzhou, China;Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China;Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China;Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Shenzhen, China;School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China;

关键词: Patchouli alcohol;    influenza virus;    antiviral;    hemagglutination;    membrane fusion;   

DOI  :  10.3389/fmicb.2022.938868

 received in 2022-06-08, accepted in 2022-12-30,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundThe antiviral activity and underlying mechanism of Patchouli alcohol remain unclear.MethodsThis study evaluated the cytotoxicity, optimal methods for drug administration, anti-influenza A activity of Patchouli alcohol. The antiviral mechanism of Patchouli alcohol was also assessed via qRT-PCR, western blot, hemagglutination inhibiting (HAI) assay, and hemolysis inhibiting assay.ResultsPatchouli alcohol was shown to have low cytotoxicity and its strongest antiviral effect was associated with premixed administration. Patchouli alcohol inhibited virus replication during the early lifecycle stages of influenza A virus infection and specifically prevented expression of the viral proteins, HA and NP. In both the HAI and hemolysis inhibiting assays, Patchouli alcohol was able to block HA2-mediated membrane fusion under low pH conditions. Patchouli alcohol had lower binding energy with HA2 than HA1.ConclusionThese findings suggest that Patchouli alcohol could be a promising membrane fusion inhibitor for the treatment of influenza A infection.

【 授权许可】

Unknown   
Copyright © 2023 Fan, Zhang, Zhang, Lai, Long, Huang, Zhan, Liu, Lai, Zhang, Pan, Su and Li.